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Research Activities

Biofilms in Human Medicine Interest Group

The goal of the Biofilms in Human Medicine Interest Group is to define the critical factors that facilitate bacterial biofilm development in human disease and then use this knowledge to develop therapies to prevent initial infection or progression to persistent biofilms (i.e. chronic and/or persistent disease).

Biofilms, which are communities of microorganisms that are attached to a surface, play a critical role in numerous infectious diseases. The CDC and the NIH recently estimated that biofilms are responsible for 65% to 80% of all infections. In 2008, the CMIB established a multi-disciplinary translational biofilms research group that consists of microbiologists, immunologists, biochemists, pathologists, computational biologist, and infectious disease experts, as well as pulmonary and otolaryngology specialists. The team meets monthly to discuss scientific and programmatic themes with the goal to submit an NIH program project grant. The human diseases represented by the projects include cystic fibrosis, chronic gastrointestinal disease, chronic otitis media and sinusitis, dental caries, infection of the genitourinary tract, periodontitis, and device related infections. Those participating include scientists and clinicians from OSU (infectious disease, dentistry, oral biology, orthopedics, microbiology, DHLRI, and FAES) as well as Nationwide Children's Hospital and Battelle Memorial Institute.

Dr Daniel Wozniak
704 Biomedical Research Tower
460 W 12th Ave
Columbus OH 43210
Email:  wozniak.1@osu.edu

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Current Funding Opportunities

1. Trainee Travel Award

2. Other Trainee Support

Monthly Meetings:  Unless otherwise noted, all meetings will be in room 105 of the Biomedical Research Tower (BRT105) on the first Wednesday of the month at 9:00am.


Participating CMIB Faculty


Daniel Wozniak, PhD

MI&I, Microbiology, ID, OSU

Brian Ahmer, PhD

Microbiology, OSU

Amal Amer, MD, PhD


Michael Bailey, PhD

Oral Biology, OSU

Lauren Bakaletz, PhD

Pediatrics, Otolaryngology, NCH

Jennifer Edwards, PhD

Pediatrics, NCH

Steven Goodman, PhD

Pediatrics, NCH

Ann Griffen, DDS, MS

Pediatric Dentistry, OSU

John Gunn, PhD

MI&I, Microbiology, ID, OSU

Luanne Hall-Stoodley, PhD


Sheryl Justice, PhD

Pediatrics, NCH

Eugene Leys, PhD

Oral Biology, OSU

Kevin Mason, PhD

Pediatrics, NCH

Abigail Norris Turner, PhD

COPH, Divison of Epidemiology, ID OSU

Vijay Pancholi, PhD

Pathology, MI&I, OSU

Paul Stoodley, PhD

MI&I, Orthopaedics, OSU

Hua Wang, PhD

Food Science & Technology, Microbiology, OSU


Participating non-CMIB Faculty


Cliff Beall, PhD

Dentistry, OSU

David Hesse

Battelle Memorial Institute

Mark Klebanoff, MD, MPH

Medicine, NCH

Purnima Kumar, PhD

Peridontology, OSU

Jiyoung Lee

COPH, Environmental Health Sciences, OSU

Steven Lower, PhD

Earth Sciences, School of Environment & Natural Resources, OSU

Sashwati Roy, PhD

Dept of Surgery, OSU

Michael Triplett, PhD

N8 Medical, Inc.

MI&I: Department of Microbial Infection and Immunity
ID: Department of Internal Medicine, Division of Infectious Diseases
PACS: Department of Internal Medicine, Division of Pulmonary, Allergy, Critical Care & Sleep Medicine
COPH: College of Public Health
OSU: The Ohio State University
NCH: Nationwide Children's Hospital

Upcoming Events

Bacteria-Material Interactions 2017 (June 14-15, 2017)

EUROBIOFILMS 2017 (September 19-22, 2017)


Lower SK, Lamlertthon S, Casillas-Ituarte NN, Lins RD, Yongsunthon R, Taylor ES, DiBartola AC, Edmonson C, McIntyre LM, Reller LB, Que YA, Ros R, Lower BH, Fowler VG Jr. Polymorphisms in fibronectin binding protein A of Staphylococcus aureus are associated with infection of cardiovascular devices. Proc Natl Acad Sci U S A. 2011 Nov 8;108(45):18372-7.


Sharpe SW, Kuehn MJ, Mason KM. Elicitation of epithelial cell-derived immune effectors by outer membrane vesicles of nontypeable Haemophilus influenzae. Infect Immun. 2011 Nov;79(11):4361-9.


Kumar PS, Matthews CR, Joshi V, de Jager M, Aspiras M. Tobacco smoking affects bacterial acquisition and colonization in oral biofilms. Infect Immun. 2011 Nov;79(11):4730-8. Epub 2011 Aug 22.


Goodman SD, Obergfell KP, Jurcisek JA, Novotny LA, Downey JS, Ayala EA, Tjokro N, Li B, Justice SS, Bakaletz LO. Biofilms can be dispersed by focusing the immune system on a common family of bacterial nucleoid-associated proteins. Mucosal Immunol. 2011 Nov;4(6):625-37. doi: 10.1038/mi.2011.27. 


Cogan NG, Gunn JS, Wozniak DJ. Biofilms and infectious diseases: biology to mathematics and back again. FEMS Microbiol Lett. 2011 Sep;322(1):1-7. doi: 10.1111/j.1574-6968.2011.02314.x. Epub 2011 Jun 16. Review. 


Byrd MS, Pang B, Hong W, Waligora EA, Juneau RA, Armbruster CE, Weimer KE, Murrah K, Mann EE, Lu H, Sprinkle A, Parsek MR, Kock ND, Wozniak DJ, Swords WE. Direct evaluation of Pseudomonas aeruginosa biofilm mediators in a chronic infection model. Infect Immun. 2011 Aug;79(8):3087-95.


Novotny LA, Clements JD, Bakaletz LO. Transcutaneous immunization as preventative and therapeutic regimens to protect against experimental otitis media due to nontypeable Haemophilus influenzae. Mucosal Immunol. 2011 Jul;4(4):456-67. 


Mason KM, Raffel FK, Ray WC, Bakaletz LO. Heme utilization by nontypeable Haemophilus influenzae is essential and dependent on Sap transporter function. J Bacteriol. 2011 May;193(10):2527-35.


Griffen AL, Beall CJ, Firestone ND, Gross EL, Difranco JM, Hardman JH, Vriesendorp B, Faust RA, Janies DA, Leys EJ. CORE: a phylogenetically-curated 16S rDNA database of the core oral microbiome. PLoS One. 2011 Apr 22;6(4):e19051.


Johnson RW, McGillivary G, Denoël P, Poolman J, Bakaletz LO. Abrogation of nontypeable Haemophilus influenzae protein D function reduces phosphorylcholine decoration, adherence to airway epithelial cells, and fitness in a chinchilla model of otitis media. Vaccine. 2011 Feb 1;29(6):1211-21.


Colvin KM, Gordon VD, Murakami K, Borlee BR, Wozniak DJ, Wong GC, Parsek MR. The pel polysaccharide can serve a structural and protective role in the biofilm matrix of Pseudomonas aeruginosa. PLoS Pathog. 2011 Jan 27;7(1):e1001264. 


Jurcisek JA, Dickson AC, Bruggeman ME, Bakaletz LO. In vitro biofilm formation in an 8-well chamber slide. J Vis Exp. 2011 Jan 20;(47). pii: 2481.


Ahmer BM, Gunn JS. Interaction of Salmonella spp. with the Intestinal Microbiota. Front Microbiol.


Lower SK. Atomic force microscopy to study intermolecular forces and bonds associated with bacteria. Adv Exp Med Biol. 2011;715:285-99. Review. 


Gonzalez-Escobedo G, Marshall JM, Gunn JS. Chronic and acute infection of the gall bladder by Salmonella Typhi: understanding the carrier state. Nat Rev Microbiol. 2011 Jan;9(1):9-14. Epub 2010 Nov 29. Review.


Alhede M, Kragh KN, Qvortrup K, Allesen-Holm M, van Gennip M, Christensen LD, Jensen PØ, Nielsen AK, Parsek M, Wozniak D, Molin S, Tolker-Nielsen T, Høiby N, Givskov M, Bjarnsholt T. Phenotypes of Non-Attached Pseudomonas aeruginosa Aggregates Resemble Surface Attached Biofilm. PLoS One. 2011;6(11):e27943. 


Lower SK, Yongsunthon R, Casillas-Ituarte NN, Taylor ES, DiBartola AC, Lower BH, Beveridge TJ, Buck AW, Fowler VG Jr. A tactile response in Staphylococcus aureus. Biophys J. 2010 Nov 3;99(9):2803-11. 

Shchipkova AY, Nagaraja HN, Kumar PS. Subgingival microbial profiles of smokers with periodontitis. J Dent Res. 2010 Nov;89(11):1247-53.


Irie Y, Starkey M, Edwards AN, Wozniak DJ, Romeo T, Parsek MR. Pseudomonas aeruginosa biofilm matrix polysaccharide Psl is regulated transcriptionally by RpoS and post-transcriptionally by RsmA. Mol Microbiol. 2010 Oct;78(1):158-72. 


Lee J, Lee CS, Hugunin KM, Maute CJ, Dysko RC. Bacteria from drinking water supply and their fate in gastrointestinal tracts of germ-free mice: a phylogenetic comparison study. Water Res. 2010 Sep;44(17):5050-8. 


Byrd MS, Pang B, Mishra M, Swords WE, Wozniak DJ. The Pseudomonas aeruginosa exopolysaccharide Psl facilitates surface adherence and NF-kappaB activation in A549 cells. MBio. 2010 Jun 29;1(3). pii: e00140-10.


Bazire A, Shioya K, Soum-Soutéra E, Bouffartigues E, Ryder C, Guentas-Dombrowsky L, Hémery G, Linossier I, Chevalier S, Wozniak DJ, Lesouhaitier O, Dufour A. The sigma factor AlgU plays a key role in formation of robust biofilms by nonmucoid Pseudomonas aeruginosa. J Bacteriol. 2010 Jun;192(12):3001-10. 


Crawford RW, Reeve KE, Gunn JS. Flagellated but not hyperfimbriated Salmonella enterica serovar Typhimurium attaches to and forms biofilms on cholesterol-coated surfaces. J Bacteriol. 2010 Jun;192(12):2981-90. Epub 2010 Jan 29. 


Crawford RW, Rosales-Reyes R, Ramírez-Aguilar Mde L, Chapa-Azuela O, Alpuche-Aranda C, Gunn JS. Gallstones play a significant role in Salmonella spp. gallbladder colonization and carriage. Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4353-8.


Borlee BR, Goldman AD, Murakami K, Samudrala R, Wozniak DJ, Parsek MR. Pseudomonas aeruginosa uses a cyclic-di-GMP-regulated adhesin to reinforce the biofilm extracellular matrix. Mol Microbiol. 2010 Feb;75(4):827-42. Epub 2010 Jan 17.