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Research Activities

Biofilms in Human Medicine Interest Group



The goal of the Biofilms in Human Medicine  Interest Group is to define the critical factors that facilitate bacterial biofilm development in human disease and then use this knowledge to develop therapies to prevent initial infection or progression to persistent biofilms (i.e. chronic and/or persistent disease).

 

Biofilms, which are communities of microorganisms that are attached to a surface, play a critical role in numerous infectious diseases. The CDC and the NIH recently estimated that biofilms are responsible for 65% to 80% of all infections. In 2008, The CMIB established a multi-disciplinary translational biofilms research group that consists of microbiologists, immunologists, biochemists, pathologists, computational biologist, and infectious disease experts, as well as pulmonary and otolaryngology specialists. The team meets monthly to discuss scientific and programmatic themes with the goal to submit an NIH program project grant. The human diseases represented by the projects include cystic fibrosis, chronic gastrointestinal disease, chronic otitis media and sinusitis, dental caries, infection of the genitourinary tract, periodontitis, and device related infections. Those participating include scientists and clinicians from OSU (infectious disease, dentistry, oral biology, orthopedics, microbiology, DHLRI, and FAES) as well as Nationwide Children's Hospital and Battelle Memorial Institute.

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Current Opportunities

1. Trainee Travel award

2. Other Trainee support

 

Members

Participating CMIB Faculty

Affiliation(s)

Daniel Wozniak, PhD

MI&I, Microbiology, ID, OSU

Brian Ahmer, PhD

Microbiology, OSU

Amal Amer, MD, PhD

MI&I, PACS, OSU

Michael Bailey, PhD

Oral Biology, OSU

Lauren Bakaletz, PhD

Pediatrics, Otolaryngology, NCH

Jennifer Edwards, PhD

Pediatrics, NCH

Steven Goodman, PhD

Pediatrics, NCH

Ann Griffen, DDS, MS

Pediatric Dentistry, OSU

John Gunn, PhD

MI&I, Microbiology, ID, OSU

Luanne Hall-Stoodley, PhD

MI&I, OSU

Sheryl Justice, PhD

Pediatrics, NCH

Eugene Leys, PhD

Oral Biology, OSU

Kevin Mason, PhD

Pediatrics, NCH

Robert Munson, PhD

Microbiology, Pediatrics, MVIMG, NCH

Abigail Norris Turner, PhD

COPH, Divison of Epidemiology, ID OSU

Vijay Pancholi, PhD

Pathology, MI&I, OSU

Paul Stoodley, PhD

MI&I, Orthopaedics, OSU

Hua Wang, PhD

Food Science & Technology, Microbiology, OSU

 

Participating non-CMIB Faculty

 

Cliff Beall, PhD

Dentistry, OSU

David Hesse

Battelle Memorial Institute

Mark Klebanoff, MD, MPH

Medicine, NCH

Purnima Kumar, PhD

Peridontology, OSU

Jiyoung Lee

COPH, Environmental Health Sciences, OSU

Steven Lower, PhD

Earth Sciences, School of Environment & Natural Resources, OSU

Sashwati Roy, PhD

Dept of Surgery, OSU

Michael Triplett, PhD

N8 Medical

MI&I: Department of Microbial Infection and Immunity
ID: Department of Internal Medicine, Division of Infectious Diseases
PACS: Department of Internal Medicine, Division of Pulmonary, Allergy, Critical Care & Sleep Medicine
MVIMG: Department of Molecular Virology, Immunology & Medical Genetics
COPH: College of Public Health
OSU: The Ohio State University
NCH: Nationwide Children's Hospital


Upcoming Events
 http://www.globaleventslist.elsevier.com/events/2015/06/european-congress-on-microbial-biofilms-eurobiofilms/

http://isom2015.com/

 7th ASM Conference on Biofilms Chicago, IL (October 24-29, 2015)

http://conferences.asm.org/index.php/upcoming-conferences/7th-asm-conference-on-biofilms

Past Events

Biofilms 6 Vienna (May 11-13, 2014)
http://biofilms6.univie.ac.at/


CTW: Biofilms in Infectious Disease: Biology to Mathematical Models and Back Again (March 22-25, 2010)

Cosponsored with Ohio State University Medical School
Organizers:
John Gunn, Dan Wozniak, and Nick Cogan


Monthly meeting schedule: First Wednesday of each month, 9:00 am

Date

Group Location
09/03/2014 Kevin Mason Lab 130 BRT
10/01/2014 Steven Lower Lab 130 BRT
11/05/2014 Ellen Gawalt (Duquesne) 165 DHLRI
12/03/2014 Alex Rickard (Michigan) 130 BRT
01/07/2015 John Gunn 130 BRT
02/04/2015 Sheryl Justice 105 BRT
03/04/2015 Kumar Lab 159 DHLRI
04/01/2015 Wozniak Lab 105 BRT
05/06/2015 Hoet Lab 105 BRT

 

Publications

  • Gunn, J.S., Marshall, J.M., Baker, S., Dongol, S., Charles, R.C., Ryan, E.T. Salmonella chronic carriage: epidemiology, diagnosis, and gallbladder persistence. Trends Microbiol. 2014 Nov;22(11): 648-55. doi: 10.1016/j.tim.2014.06.007. Epub 2014 Jul 22. PudMed PMID: 25065707; PubMed Central PMCID: PMC4252485. 
  • Limoli, D.H., Rockel, A.B., Host, K.M., Jha, A., Kopp, B.T., Hollis, T., Wozniak, D.J. Cationic antimicrobial peptides promote microbial mutagenesis and pathoadaptation in chronic infections. PLOS Pathog. 2014 Apr 24;10(4):e1004083. doi: 10.1371/journal.ppat.1004083. eCollection 2014 Apr. 
  • Jones, C.J., Newsom, D., Kelly, B., Irie, Y., Jennings, L.K., Xu, B., Limoli, D.H., Harrison, J.J., Parsek, M.R., White, P., Wozniak, D.J. ChIP-Seq and RNA-Seq reveal an AmrZ-mediated mechanism for cyclic di-GMP synthesis and biofilm development by Pseudomonas aeruginosa. PLOS Pathog. 2014 Mar 6;10(3):e1003984. doi: 10.1371/journal.ppat.1003984. eCollection 2014 Mar.
  • Doroshenko, N., Tseng, B.S., Howlin, R.P., Deacon, J., Parsek, M.R., Wharton, J., Thurner, P.J., Gilmore, B.F., Stoodley, P. 2014. Extracellular DNA impedes the transport of vancomycin in Staphylococcus epidermidis biofilms pre-exposed to sub-inhibitory concentrations of vancomycin. Antimicrob. Agents Chemo. 58(12):7273-82. 
  • McConoughey, S.J., Howlin, R.P., Granger, J.F., Manring, M.M., Calhoun, J.H., Shirtliff, M., Kathju, S., Stoodley, P. 2014. Biofilms in periprosthetic orthopaedic infections. Future Microbiol. 9:987-1007.
  • Schaudinn, C., Stoodley, P., Hall-Stoodley, L., Gorur, A., Remis, R., Auer, M., Hertwig, S., Wu, S., Guerrero-Given, D., Ze Hu, F., Ehrlich, G., Costerton, J.W., Webster, P. 2014. Death and transfiguration in static Staphylococcus epidermidis cultures. PLOS One 9:6, e100002. 
  • Lappin-Scott, H., Burton, S., Stoodley, P. 2014. Revealing a world of biofilms - the pioneering research of Bill Costerton. Nat. Revs. Microbiol. 12(11):781-7. 
  • McConoughey, S.J., Howlin, R.P., Wiseman, J., Stoodley, P., Calhoun, J.H. 2014. Comparing PMMA and calcium sulfate as carriers for the local delivery of antibiotics to infected surgical sites. Journal of Biomedical Materials Research: Part B-Applied Biomaterials. Aug 20. doi: 10.1002/jbm.b.33247. [Epub ahead of print]. PMID: 25142105.
  • Jhass, A.K., Johnston, D.A., Gulati, A., Anand, R., Stoodley, P.* Sharma, S. 2014. A scanning electron microscope characterisation of biofilm on failed craniofacial osteosynthesis miniplates. Journal of Cranio-Maxillofacial Surgery. 42(7):e372-8. 
  • Kathju, S., Nistico, L., Tower, I., Lasko, L-A., Stoodley, P. 2014. Bacterial Biofilms on Implanted Suture Material are a Cause of Surgical Site Infection. Surg Infect. 15(5):592-600.
  • Nistico, L., Hall-Stoodley, L., Stoodley, P. 2014. Imaging Bacteria and Biofilms on Hardware and Periprosthetic Tissue in Orthopedic Infections. In Microbial Biofilms Donelli G (Ed.). Methods Mol Biol. 1147:105-26. 
  • Grande, R., Nistico, L., Sambanthamoorthy, K., Longwell, M., Lannitelli, A., Luigina, C., di Stefano, A., Hall-Stoodley, L., Stoodley, P. 2014. Temporal expression of agrB, cidA and alsS in the early development of Staphylococcus aureus UAMS-1 biofilm formation and the structural role of extracellular DNA and carbohydrates. FEMS Pathogens and Disease. 70(3): 414-22. 
  • Francolini, I., Donelli, G., Vuotto, C., Baroncini, F., Stoodley, P., Taresco, V., Martinelli, A., D'llario, L., Piozzi, A. 2014. Antifouling polyurethanes to fight device-related staphylococcal infections: synthesis, characterization and antibiofilm efficacy. FEMS Pathogens and Disease. 70(3):401-7. 
  • Lourenco, A., Coenye, T., Goeres, D., Donelli, G., Azevedo, A., Ceri, H., Coelho, F.L., Flemming, H-C., Juhna, T., Lopes, S.P., Oliveira, R., Oliver, A., Shirtliff, M.E., Sousa, A.M., Stoodley, P., Pereira, M.O., Azevedo, N.F. 2014. Minimum information about a biofilm experiment (MIABIE): standards for reporting experiments and data on sessile microbial communities living at interfaces. FEMS Pathogens and Disease. 70(3):250-6.
  • Elgharably, H., Mann, E., Awad, H., Ganesh, K., Ghatak, P.D., Gordillo, G., Sai-Sudhakar, C.B., Roy, S., Wozniak, D.J., Sen, C.K. First evidence of sternal wound biofilm following cardiac surgery. PLOS One. 2013 Aug 1;8(8):e70360. doi: 10.1371/journal.pone.0070360. Print 2013.  
  • Wang, S., Parsek, M.R., Wozniak, D.J., Ma, L.Z. A spider web strategy of type IV pili-mediated migration to build a fibre-like Psl polysaccharide matrix in Pseudomonas aeruginosa biofilms. Environ Microbiol. 2013 Aug;15(8):2238-53. doi:10.1111/1462-2920.12095. Epub 2013 Feb 20. 
  • Eguale, T., Marshall, J., Molla, B., Bhatiya, A., Gebreyes, W.A., Engidawork, E., Asrat, D., Gunn, J.S. Association of multicellular behaviour and drug resistance in Salmonella enterica serovars isolated from animals and humans in Ethiopia. J Appl Microbiol. 2014 Oct; 117(4):961-71. doi: 10.1111/jam.12579. Epub 2014 Jul 7. 
  • Brockson, M.E., Novotny, L.A., Morzan, E.M., Malhotra, S., Jurcisek, J.A., Akbar, R., Devaraj, A., Goodman, S.D., Bakaletz, L.O. (2014). Evaluation of the kinetics and mechanism of action of anti-integration host factor mediated disruption of bacterial biofilms. Molecular Microbiology. 93: 1246-1258.
  • Howlin, R.P., Brayford, M.J., Webb, J.S., Cooper, J.J., Aiken, S.S., Stoodley, P. 2015. Antibiotic-loaded synthetic calcium sulfate beads for the prevention of bacterial colonisation and biofilm formation in periprosthetic infections. Antimicrob. Agents Chemo. 59(1), 111-120. 
  • Otter, J.A., Vickery, K., Walker, J., deLancey Pulcini, E., Stoodley, P., Salkeld, J., Chewins, J., Yezli, S., Edgeworth, J.D. 2015. Surface-attached cells, biofilms and biocide susceptibility: implications for hospital cleaning and disinfection Journal of Hospital Infection. J Hosp Infect. 89(1), 16-27. 
  • Wozniak, D.J., Parsek, M.R. Surface-associated microbes continue to surprise us in their sophisticated strategies for assembling biofilm communities. F1000Prime Rep. 2014 May 6;6:26. doi: 10.12703/P6-26. eCollection 2014. Review.
  • Roy, S., Elgharably, H., Sinha, M., Ganesh, K., Chaney, S., Mann, E., Miller, C., Khanna, S., Bergdall, V.K., Powell, H.M., Cook, C.H., Gordillo, G.M., Wozniak, D.J., Sen, C.K. Mixed-species biofilm compromises wound healing by disrupting epidermal barrier function. J Pathol. 2014 Aug;233(4):331-43. doi: 10.1002/path.4360. Epub 2014 May 27. 
  • Marshall, J.M., Flechtner, A.D., La Perle, K.M., Gunn, J.S. Visualization of extracellular matrix components within sectioned Salmonella biofilms on the surface of human gallstones. PLOS One. 2014 Feb 14;9(2):e89243. doi: 10.1371/journal.pone.0089243. eCollection 2014. 
  • Beall, C.J., Campbell, A.G., Dayeh, D.M., Griffen, A.L., Podar, M., Leys, E.J. Single cell genomics of uncultured, health-associated Tannerella BU063 (oral taxon 286) and comparison to the closely related pathogen Tannerella forsythia. PLOS One. 2014 Feb 14;9(2):e89398. doi: 10.1371/journal.pone.0089398. eCollection 2014. 
  • Wang, S., Liu, X., Liu, H., Zhang, L., Guo, Y., Yu, S., Wozniak, D.J., Ma, L.Z. The exopolysaccharide Psl-eDNA interaction enables the formation of a biofilm skeleton in Pseudomonas aeruginosa. Environ Microbiol Rep. 2014 Dec 4. doi: 10.1111/1758-2229.12252. [Epub ahead of print].
  • Roy, S., Elgharably, H., Sinha, M., Genesh, K., Chaney, S., Mann, E., Miller, C., Khanna, S., Bergdall, V.K., Powell, H.M., Cook, C.H., Gordillo, G.M., Wozniak, D.J., Sen, C.K. Mixed-species biofilm compromises wound healing by disrupting epidermal barrier function. J Pathol. 2014 Aug; 233(4):331-43. doi: 10.1002/path.4360. Epub 2014 May 27.
  • Elgharably, H., Mann, E., Awad, H., Ganesh, K., Ghatak, P.D., Gordillo, G., Sai-Sudhakar, C.B., Roy, S., Wozniak, D.J., Sen, C.K. First evidence of sternal wound biofilm following cardiac surgery. PLOS One. 2013 Aug 1;8(8):e70360. doi: 10.1371/journal.pone.0070360. Print 2013.
  • Cho, C., Chande, A., Gakhar, L., Bakaletz, L.O., Jurcisek, J.A., Ketterer, M., Shao, J., Gotoh, K., Hunt, J., O’Brien, E., Apicella, M.A.  (2014). Role of the nuclease of nontypeable Haemophilus influenzae in dispersal of organisms from biofilms.  Infect Immun.  Epub ahead of print 12/29/14.
  • Hoiby, N., Bjarnsholt, T., Moser, C., Bassi, G.L., Coenye, T., Donelli, G., Hall-Stoodley, L., Hola, V., Imbert, C., Kirketerp-Moller, K., Lebeaux, D., Oliver, A., Ullmann, A.J., Williams, C., for the ESCMID* Study Group for Biofilms (ESGB) and Consulting External Expert Werner Zimmerli. 2015. ESCMID guideline for the diagnosis and treatment of biofilm infections 2014. Clinical Microbiology Infection. In Press. Available online 14 January 2015. doi: 10.1016/j.cmi.2014.10.024 *European Society of Clinical Microbiology and Infectious Diseases.
  • Wilkins, M., Hall-Stoodley, L., Allan, R.N., Faust, S.N. 2014 New approaches to the treatment of biofilm-related infections. J Infect. Nov;69 Suppl 1:S47-52. doi: 10.1016/j.jinf.2014.07.014. Epub 2014 Sep 17.  
  • Allan, R., Skipp, P., Jeffries, J., Clarke, S., Faust, S., Hall-Stoodley, L.,* Webb, J.* 2014. Pronounced Metabolic Changes in Adaptation to Biofilm Growth by Streptococcus pneumonia. PLOS One. 9(9):e107015.
  • Nistico, L., Hall-Stoodley, L., Stoodley, P. 2014. Imaging bacteria and biofilms on hardware and periprosthetic tissue in orthopedic infections. Methods Mol Biol. 1147:105-26. doi:10.1007/978-1-4939-0467-9_8.
  • Walker, W.T., Jackson, C.L., Coles, J., Lackie P., Faust, S.N., Hall-Stoodley, L., Lucas, J.S. 2014. Ciliated Cultures From Patients With Primary Ciliary Dyskinesia Produce Nitric Oxide in Response to Haemophilus influenzae Infection and Proinflammatory Cytokines. Chest. 145(3): 668-669. doi:10.1378/chest. 13-2398.
  • Grande, R., Nistico, L., Sambanthamoorthy, K., Longwell, M., Iannitelli, A., Cellini, L., Di Stefano, A., Hall-Stoodley, L., Stoodley, P. 2014. Temporal expression of agrB, cidA and alsS in the early development of Staphylococcus aureus UAMS-1 biofilm formation and the structural role of extracellular DNA and carbohydrates. Pathog Dis. Feb 17. doi: 10.1111/2049-632X. 12158. [Epub ahead of print]
  • Schaudinn, C., Stoodley, P., Hall-Stoodley, L., Gorur, A., Remis, J., Wu, S., Auer, M., Hertwig, S., Guerrero-Given, D., Hu, F.Z., Ehrlich, G., Costerton, J., Robinson, D., Webster, P. 2014. Death and transfiguration in static Staphylococcus epidermidis Cultures.  PLOS One. 9(6):e100002.
     
  • Brockson, M.E., Novotny, L.A., Mokrzan, E.M., Malhotra, S., Jurcisek, J.A., Akbar, R., Devaraj, A., Goodman, S.D., Bakaletz, L.O. (2014).  Evaluation of the kinetics and mechanism of action of anti-integration host factor mediated disruption of bacterial biofilms.  Mol Microbiol, 93(6):1246-1258.  PMCID:PMC4160410. 
     
  • Das, S., Rosas, L.E., Jurcisek, J.A., Novotny, L.A., Bakaletz, L.O. (2014). Improving patient care via development of a protein-based diagnostic test for microbe-specific detection of chronic rhinosinusitis.  Laryngoscope, 124(3):605-615.  PMCID:PMC4020599
     
  • Novotny, L.A., Clements, J.D., Bakaletz, L.O. (2013).  Kinetic analysis and evaluation of the mechanisms involved in the resolution of experimental nontypeable Haemophilus influenzae-induced otitis media after transcutaneous immunization.  Vaccine, 31(34):3417-3426.  PMCID: PMC3578125.
     
  • Brandstetter, K.A., Jurcisek, J.A., Goodman, S.D., Bakaletz, L.O., Das, S. (2013).  Antibodies directed against integration host factor mediate biofilm clearance from Nasopore®.  Laryngoscope, 123(11):2626-2632.
     
  • Novotny, L.A., Amer, A.O., Goodman, S.D., Bakaletz, L.O. (2013).  Structural stability of Burkholderia cenocepacia biofilms is reliant on eDNA structure and presence of a bacterial nucleic acid binding protein.  PLoS One, 8(6):e67629.  PMCID:PMC3682984.
     
  • Abu Khweek, A., Fernandez Davila, N., Caution, K., Akhter, A., Abdulrahman, B., Tazi, M., Hassan, H., Novotny, L.A., Bakaletz, L.O., Amer, A.O. (2013).  Biofilm-derived Legionella pneumophila evades the innate immune response in macrophages.  Front Cell Infect Microbiol, 27(3):18.  PMCID:PMC3664316.
     
  • Gustave, J.E., Jurcisek, J.A., McCoy, K.S., Goodman, S.D., Bakaletz, L.O.  (2013). Targeting bacterial integration host factor to disrupt biofilms associated with cystic fibrosis. J Cystic Fibrosis, 12(4):384-389. PMCID: PMC3582735.
     
  • Jones, E.A., McGillivary, G., Bakaletz, L.O. (2013).  Extracellular DNA with a nontypeable Haemophilus influenzae induced biofilm serves as a sink for cationic host defense peptides.  J Innate Immun, 5(1):24-38.  PMCID: PMC3640559.
     
  • Brockson, M.E., Jurcisek, J.A., McGillivary, G., Bowers, M.R., Bakaletz, L.O. (2012).  Respiratory syncytial virus promotes Moraxella catarrhalis-induced ascending experimental otitis media.  PLoS One, 7(6):e40088.  PMCID:PMC3387005.
     
  • Bakaletz, L.O. (2012).  Bacterial biofilms in the airway – evidence for role in pathology and implications for treatment.  Paediatr Respir Rev, 13(3):154-159.  PMCID:PMC3509202.
  • Christian M. Harding, Erin N. Tracy, Michael D. Carruthers, Philip N. Rather, Luis A. Actis, Robert S. Munson, Jr.  Acinetobacter baumannii Strain M2 Produces Type IV Pili Which Play a Role in Natural Transformation and Twitching Motility but Not Surface-Associated Motility. mBio, July/August 2013; 4(4): e00360-13.

  • Lower SK, Lamlertthon S, Casillas-Ituarte NN, Lins RD, Yongsunthon R, Taylor ES, DiBartola AC, Edmonson C, McIntyre LM, Reller LB, Que YA, Ros R, Lower BH, Fowler VG Jr.

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