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Sam King, PhD


Staff photo

700 Childrens Dr
Ctr for Microbial Pathogenesis
Columbus, OH 43205

Phone: (614) 722-2915

Email: king.1058@osu.edu

 

Current OSU Appointment

Associate Professor, Pediatrics

 

Biographical

In 1994 I obtained my undergraduate (BS, honors) degree from Leicester University (United Kingdom). I then moved to the University of Warwick (United Kingdom) to complete my PhD, where I received a fellowship from the Biotechnology and Biological Sciences Research Council (BBSRC) and SmithKline Beecham to study with Dr. Chris Dowson. My thesis research was focused on the molecular epidemiology of pneumococcal neuraminidases. Following the award of my PhD in 1999 I completed a short Post-doctoral Fellowship with Dr. Adrian Whatmore, developing a typing scheme and studying molecular epidemiology of Streptococcus suis. Following this I moved to The University of Pennsylvania where I completed a Postdoctoral Fellowship studying the ability of pneumococci to modify host glycoconjugates with Dr. Jeff Weiser.

In the fall of 2005 I started as an Assistant Professor at Ohio State University and The Research Institute at Nationwide Children's Hospital (TRINCH). Since this time I have become internationally recognized as an expert in pneumococcal modification of host glycans and the consequences of this modification to pathogenesis. We are now expanding our research interests to encompass pneumococcal viral synergy, the pathogenic mechanisms of pneumococcal hemolytic uremic syndrome and nanoparticle vaccines.

Academic Advising

2008 - present , The Ohio State University.
2016 Mercedes Nikzad, The Ohio State University. Graduated 0.
2016 Jasmin Carmona, The Ohio State University. Graduated 0.
 

Chapters in Books

King, Samantha J.. "Advances in Vaccines for Streptococcus pneumoniae." In XIAPO Manual of Pediatric Otorhinolaryngology. Edited by Sih, T., Chinski, A., Eavey, R., and Goddino, R.. 225-248. Sao Paulo, BR|BRA: Editora e Grafica Vida & Consciencia, October 2011.

 

Degrees

1994 None Indicated, University of Leicester

1999 Ph.D., University of Warwick

 

Editorial Activities

2004 - present Infection and Immunity
2005 - present Scandinavian Journal of Infectious Disease
2006 - present Journal of Bacteriology
2006 - present JARO
2007 - present Clinical Vaccine and Immunology
2007 - present Microbial Pathogenesis
2008 - present Trends in Microbiology
2008 - present Journal of Clinical Microbiology
2008 - present PLoS Pathogens
2008 - present PLoS ONE
2008 - present Journal of Infectious Diseases
2008 - present FEMS Microbiology Letters
2008 - present Expert Review of Anti-infective Therapy
2009 - present Molecular Microbiology
2009 - present FEMS Immunology and Medical Microbiology
2010 - present Antimicrobial Agents in Chemotherapy
2010 - present Frontiers In Cellular Microbiology
2010 - 2012 Infection and Immunity
2011 - present Applied and Environmental Microbiology
2011 - present Structure
2011 - present Journal of Medical Microbiology
2011 - present Journal of Innate Immunity
2011 - present mBio
2012 - present Folia Microbiologica
2012 - present BMC Microbiology
2012 - present The Journal of Pediatrics
2012 - present Molecular Oral Microbiology
2013 - present European Journal of Clinical Microbiology & Infectious Diseases
2013 - present Biochemistry
2014 - present Frontiers in Veterinary Infectious Diseases
2015 Journal of Antimicrobial Chemotherapy
2015 Microbiology
2016 - present MicrobiologyOpen
2016 - present Virulence
2016 - present Frontiers in Cellular and Infection Microbiology
2016 - present Froniters in Physiology
 

Honors

2017 - present Outstanding Mentor Award for Basic Research, 2016. The Research Institute at Nationwide Children's Hospital.
 

Journal Articles

Dowson, C. G., Barcus, V., King, S. J., Pickerill, P., Whatmore, A. M. and Yeo, M.. "Horizontal gene transfer and the evolution of resistance and virulence determinants of Streptococcus.." Journal of Applied Microbiology. Vol. 83, (January 1997.): 42S-51S.

Dowson C.G., Barcus V., King S.J., Pickerill P., Whatmore A. and Yeo M.. "Horizontal gene transfer and the evolution of resistance and virulence determinants in Streptococcus." Journal of Applied Microbiology. Vol. 83, (January 1997.): S42-S51.

Whatmore, A. M., King, S. J., Doherty, N. C., Sturgeon, D., Chanter, N. and Dowson, C. G.. "Molecular characterization of equine isolates of Streptococcus pneumoniae: natural disruption of genes encoding the virulence factors pneumolysin and autolysin.." Infection and Immunity. Vol. 67, (January 1999.): 2776-2782.

Muller-Graf, C., Whatmore, A., King, S. J., Pickerill, A. P., Doherty, N., Paul, J., Griffiths, D., Crook, D. and Dowson, C. G.. "Population biology of Streptococcus pneumoniae isolated from oropharyngeal carriage and invasive disease.." Microbiology. Vol. 145, (January 1999.): 2776-2782.

Whatmore A.M., King S.J., Doherty N.C., Sturgeon D., Chanter N. and Dowson C.G.. "Molecular characterization of equine isolates of Streptococcus pneumoniae: Natural disruption of genes encoding the virulence factors pneumolysin and autolysin." Infection and Immunity. Vol. 67, no. 6. (June 1999.): 2776-2782.

Muller-Graf C.DM., Whatmore A.M., King S.J., Trzcinski K., Pickerill A.P., Doherty N., Paul J., Griffiths D., Crook D. and Dowson C.G.. "Population biology of Streptococcus pneumoniae isolated from oropharyngeal carriage and invasive disease." Microbiology- SGM. Vol. 145, (November 1999.): 3283-3293.

King, S. J., Heath, P., Luque, I., Tarradas, C., Dowson, C. G. and Whatmore, A. M.. "Distribution and genetic diversity of suilysin in Streptococcus suis isolated from different diseases of pigs: characterization of the genetic basis of suilysin absence in isolates frequently associated with pneumonia.." Infection and Immunity. Vol. 69, (January 2001.): 7572-7582.

King S.J., Heath P.J., Luque I., Tarradas C., Dowson C.G. and Whatmore A.M.. "Distribution and genetic diversity of suilysin in Streptococcus suis isolated from different diseases of pigs and characterization of the genetic basis of suilysin absence." Infection and Immunity. Vol. 69, no. 12. (December 2001.): 7572-7582.

Shakhnovich E.A., King S.J. and Weiser J.N.. "Neuraminidase expressed by Streptococcus pneumoniae desialylates the lipopolysaccharide of Neisseria meningitidis and Haemophilus influenzae: a paradigm for interbacterial competition among pathogens of the human respiratory tract." Infection and Immunity. Vol. 70, no. 12. (December 2002.): 7161-7164.

King, S. J., Leigh J. A., Heath P. J., Luque I., Tarradas C., Dowson C. G., Whatmore A. M.. "Development of a multilocus sequence typing scheme for the pig pathogen Streptococcus suis: identification of virulent clones and potential capsular serotype exchange.." Journal of Clinical Microbiology. Vol. 40, (January 2002.): 3671-3680.

Shakhnovich E. A., King, S. J., and Weiser, J. N.. "Neuraminidase expressed by Streptococcus pneumoniae desialylates the lipopolysaccharide of Neisseria meningitidis and Haemophilus influenzae: A paradigm for interbacterial competition among pathogens of the human respiratory tract.." Infection and Immunity. Vol. 70, (January 2002.): 7161-7164.

King S.J., Leigh J.A., Heath P.J., Luque I., Tarradas C., Dowson C.G. and Whatmore A.M.. "Development of a multilocus sequence typing scheme for the pig pathogen Streptococcus suis: Identification of virulent clones and potential capsular serotype exchange." Journal of Clinical Microbiology. Vol. 40, no. 10. (October 2002.): 3671-3680.

King S.J., Allen A.G., Maskell D.J., Dowson C.G. and Whatmore A.M.. "Distribution, genetic diversity, and variable expression of the gene encoding hyaluronate lyase within the Streptococcus suis population." Journal of Bacteriology. Vol. 186, no. 14. (July 2004.): 4740-4747.

King S. J., Hippe K. R., Gould J. M., Bae D., Peterson S., Cline R. T., Fasching, C., Janoff E. N., and Weiser J. N.. "Phase variable desialylation of host proteins that bind to Streptococcus pneumoniae in vivo and protect the airway.." Molecular Microbiology. Vol. 54, (January 2004.): 159-171.

King S.J., Hippe K.R., Gould J.M., Bae D., Peterson S., Cline R.T., Fasching C., Janoff E.N. and Weiser J.N.. "Phase variable desialylation of host proteins that bind to Streptococcus pneumoniae in vivo and protect the airway." Molecular Microbiology. Vol. 54, no. 1. (October 2004.): 159-171.

King, S. J., Allen, A. G., Maskell, D. J., Dowson, C. G. and Whatmore, A. M.. "Distribution, genetic diversity, and variable expression of the gene encoding hyaluronate lyase within the Streptococcus suis population.." Journal of Bacteriology. Vol. 186, (January 2004.): 4740-4747.

King S.J., Whatmore A.M. and Dowson C.G.. "NanA, a neuraminidase from Streptococcus pneumoniae, shows high levels of sequence diversity, at least in part through recombination with Streptococcus oralis." Journal of Bacteriology. Vol. 187, no. 15. (August 2005.): 5376-5386.

King, S. J., Whatmore, A. M., Dowson, C. G.. "NanA, a neuraminidase from Streptococcus pneumoniae, shows high levels of sequence diversity, at least in part through recombination with Streptococcus oralis.." Journal of Bacteriology. Vol. 187, (January 2005.): 5376-5786.

King S.J., Hippe K.R. and Weiser J.N.. "Deglycosylation of human glycoconjugates by the sequential activities of exoglycosidases expressed by Streptococcus pneumoniae." Molecular Microbiology. Vol. 59, no. 3. (February 2006.): 961-974.

King, S. J., Hippe K. R., and Weiser J. N.. "Deglycosylation of human glycoconjugates by the sequential activities of exoglycosidases expressed by Streptococcus pneumoniae. Molecular Microbiology." Molecular Microbiology. Vol. 59, (January 2006.): 961-974.

Roche A.M., King S.J. and Weiser J.N.. "Live attenuated Streptococcus pneumoniae strains induce serotype-independent mucosal and systemic protection in mice." Infection and Immunity. Vol. 75, no. 5. (May 2007.): 2469-2475.

Roche A. M., King, S. J., Weiser J. N.. "Live attenuated Streptococcus pneumoniae strains induce serotype-independent mucosal and systemic protection in mice." Infection and Immunity. Vol. 75, (January 2007.): 2469-2475.

Gut H., King S.J. and Walsh M.A.. "Structural and functional studies of Streptococcus pneumoniae neuraminidase B: An intramolecular trans-sialidase." FEBS Letters. Vol. 582, no. 23-24. (October 2008.): 3348-3352.

Burnaugh A. M., Frantz L. J., King, S. J.. "Growth of Streptococcus pneumoniae on human glycoconjugates is dependent upon the sequential activity of bacterial exoglycosidases." The Journal of Bacteriology. Vol. 190, (January 2008.): 221-230.

Gut, H., King, S. J., and Walsh, M. A.. "Structural and functional studies of Streptococcus pneumoniae neuraminidase B: an intramolecular transsialidase." FEBS Letters. Vol. 582, (January 2008.): 3348-3352.

Burnaugh A.M., Frantz L.J. and King S.J.. "Growth of Streptococcus pneumoniae on human glycoconjugates is dependent upon the sequential activity of bacterial exoglycosidases." Journal of Bacteriology. Vol. 190, no. 1. (January 2008.): 221-230.

Marion C., Limoli D.H., Bobulsky G.S., Abraham J.L., Burnaugh A.M. and King S.J.. "Identification of a pneumococcal glycosidase that modifies O-linked glycans." Infection and Immunity. Vol. 77, no. 4. (April 2009.): 1389-1396.

Marion, C., Limoli, D. H., Bobulsky, G. H., Abraham, J. L., Burnaugh, A. M., and King, S. J.. "Identification of a pneumococcal O-glycosidase that modifies O-linked glycans." Infection and Immunity. Vol. 77, no. 4. (January 2009.): 1389-1396.

King S.J.. "Pneumococcal modification of host sugars: a major contributor to colonization of the human airway?." Molecular Oral Biology. Vol. 25, no. 1. (February 2010.): 15-24.

King, S. J.. "Pneumococcal modification of host sugars: a major contributor to colonization of the human airway?." Molecular Oral Microbiology. Vol. 25, no. 1. (February 2010.): 15-.

Marion,Carolyn; Burnaugh,Amanda,M; Woodiga,Shireen,A; King,Samantha,J. "Sialic Acid Transport Contributes to Pneumococcal Colonization." INFECTION AND IMMUNITY. Vol. 79, no. 3. (March 2011.): 1262-1269.

Marion C., Burnaugh A. M., Woodiga S. A., King S. J.. "Sialic acid transport contributes to pneumococcal colonization." Infection and Immunity. Vol. 79, no. 3. (March 2011.): 1262-1269.

Chen G., Chen X., King S., Cavassani K.A., Cheng J., Zheng X., Cao H., Yu H., Qu J., Fang D., Wu W., Bai X., Liu J., Woodiga S., Chen C., Sun L., Hogaboam C.M., Kunkel S.L., Zheng P and Liu Y.. "Disruption of Sialic Acid-dependent Pattern Recognition Exacerbates Polybacterial Sepsis." Nature Biotechnology. no. 29. (January 2011.): 428-435.

Limoli D.H., Sladek J.A., Fuller L.A., Singh A.K. and King S.J.. "BgaA acts as an adhesin to mediate attachment of some pneumococcal strains to human epithelial cells." Microbiology- SGM. Vol. 157, (August 2011.): 2369-2381.

Marion C., Burnaugh A.M., Woodiga S.A. and King S.J.. "Sialic acid transport contributes to pneumococcal colonization." Infection and Immunity. Vol. 79, no. 3. (March 2011.): 1262-1269.

Marion C., Aten A.E., Woodiga S.A. and King S.J.. "Identification of an ATPase, MsmK, which energizes multiple carbohydrate ABC transporters in Streptococcus pneumoniae." Infection and Immunity. Vol. 79, no. 10. (October 2011.): 4193-4200.

Marion, C., Aten, A.E., Woodiga, S.A. and King, S.J.. "Identification of an ATPAse, MsmK, which energizes multiple carbohydrate ABC transporters in Streptococcus pneumoniae." Infection and Immunity. Vol. 10, no. 79. (January 2011.): -.

Limoli, D.H., Sladek, J.A., Fuller, L.A., Singh, A.K. and King, S.J.. "BgaA acts as an adhesin to mediate attacement of some pneumococcal strains to human epithelial cells.." Microbiology. Vol. 8, no. 157. (January 2011.): 2369-2381.

Chen G.Y., Chen X., King S.J., Cavassani K.A., Cheng J., Zheng X., Cao H., Yu H., Qu J., Fang D., Wu W., Bai X.F., Liu J.Q., Woodiga S.A., Chen C., Sun L., Hogaboam C.M., Kunkel S.L., Zheng P. and Liu Y.. "Amelioration of sepsis by inhibiting sialidase-mediated disruption of the CD24-SiglecG interaction." Nature Biotechnology. Vol. 29, no. 5. (May 2011.): 428-435.

Buckwalter C.M. and King S.J.. "Pneumococcal carbohydrate transport: food for thought." Trends in Microbiology. Vol. 20, no. 11. (September 2012.): 517-522.

Marion C., Stewart J.M., Tazi M.F., Burnaugh A.M., Linke C.M., Woodiga S.A. and King S.J.. "Streptococcus pneumoniae can utilize multiple sources of hyaluronic acid for growth." Infection and Immunity. Vol. 80, no. 4. (April 2012.): 1390-1398.

Linke C.M., Woodiga S.A., Meyers D.J., Buckwalter C.M., Salhi H.E. and King, S.J.. ") The ABC transporter encoded at the pneumococcal fructooligosaccharides utilization locus correlates with the ability to utilize long and short chain fructooligosaccharides." Journal of Bacteriology. Vol. 195, no. 5. (March 2013.): 1031-1041.

Link C.M., Woodiga S.A., Meyers D.J., Buckwalter C.M., Salhi H.E., and King S.J. "The ABC Transporter Encoded at the Pneumococcal Fructooligosaccharide Utilization Locus Determines the Ability To Utilize Long- and Short-Chain Fructooligosaccharides." Journal of Bacteriology. Vol. 195, no. 5. (March 2013.): 1031-1041.

Ouyang K., Woodiga S., Dwivedi V., Marion C., Singh A., Binjawadagi B., Hiremath J., Manickam C., Schleappi R., Mahesh K., Wu J., King S., and Renukaradhya G.J.. "Pretreatment of epithelial cells with live Streptococcus pneumoniae has no detectable effect on influenza A virus replication in vitro." PLoS One. Vol. 9, no. 3. (March 2014.): e90066-.

Singh A.K., Pluvinage B., Higgins M.A., Dalia A.B., Woodiga S.A., Flynn M., Lloyd A.R., Wesier J.N., Stubbs K.A., Boraston A.B., and King S.J.. "Unravelling the multiple functions of the architecturally intricate Streptococcus pnumoniae beta-galactosidase, BgaA." PLoS Pathogens. Vol. 10, no. 9. (September 2014.): e1004364-.

Singh A.K., Osman A.S., Woodiga S.A., White P., Mahan J.D., and King S.J.. "Defining the role of pneumococcal neuraminidases and O-glycosidase in pneumococcal hemolytic uremic syndrome." Journal of Medical Microbiology. Vol. Epub ahead of print, (July 2016.): -.

Sundaram S., Rahman M.A., Mitra S., Woodiga S.A., King S.J. and Wewers M.D.. "IκBϚ regulates human monocyte-pro-inflammatory responses induced by Streptococcus pneumoniae." PLoS One. Vol. 11, no. 9. (September 2016.): e0161931-.

Singh A.K., Woodiga S.A., Grau M. and King S.J.. "Streptococcus oralis binds platelets via sequential adherence to host glycans." Infection and Immunity. Vol. Doi:10.1128/IAI.00774-16, (December 2016.): Epub ahead of print-.

Blanchette K.A., Shenoy A.T., Milner J.2nd, Gilley R.P., McClure E., Hinojosa C.A., Kumar N., Daughtery S.C., Tallon L.J., Ott S., King S.J., Ferreira D.M., Gordon S.B., Tettelin H., and Orihuela C.J.. "Neuraminidase: A exposed galactose promotes Streptococcus pneumoniae biofilm formation during colonization." Infection And Immunity. Vol. Epub ahead of print, (August 2016.): .-.

Culurgiona S., Harris G., Singh A.K., King S.J. and Walsh M.A.. "Structrual basis for regulation and specificity of fructooligosaccharide import in Streptococcus pneumoniae." Structure. Vol. 15, (January 2017.): 1-15.

Robb M., Hobbs J.K., Woodiga S.A., Shapir-Ward S., Suits M.D.L., McGregor N., Brumer H., Yesilkaya H., King S.J. and Boraston A.B.. "Molecular characterization of N-glycan degradation and transport in Streptococcus pneumonia and its contribution to virulence." PLoS Pathogens. Vol. 13, no. 1. (January 2017.): e1006090-.

Robb N., Hobbs J.K., Woodiga S.A., Shapiro-Wardes S., Suits M.D.L., McGregor N., Brumer H., Yesilkaya H., King S.J. and Boraston A.B.. "Molecular characterization of N-glycan degradation and transport in Streptococcus pneumoniae and its contribution to virulence." PLoS Pathogens. -.

Linke C.M., Woodiga S.A., Meyers D.J., Buckwalter C.M., Salhi H.E. and King S.J.. "The ABC transporter encoded at the pneumococcal fructooligosaccharides utilization locus correlates with the ability to utilize long and short chain fructooligosaccharides." Journal of Bacteriology. -.

 

Presentations

"Bacterial deglycosylation of host proteins that protect the human airway." Presented at The Research Institute at Nationwide Children's Hospital, Columbus, OH, US|USA. (October 2004)

"Bacterial deglycosylation of host proteins that protect the human airway." Presented at Division of Biological Sciences, Section of Microbiology, University of California Davis, Davis, CA, US|USA. (January 2005)

"Bacterial deglycosylation of host proteins that protect the human airway." Presented at Department of Biomedical Sciences, University of Maryland Dental School, Baltimore, MD, US|USA. (January 2005)

"Bacterial deglycosylation of host proteins that protect the human airway." Presented at Department of Biology, University of Texas at San Antonio, San Antonio, TX, US|USA. (March 2005)

"Bacterial deglycosylation of host proteins that protect the human airway." Presented at Department of Cell Biology and Molecular Biology, University of Maryland, Baltimore, MD, US|USA. (February 2005)

"Bacterial deglycosylation of host proteins that protect the human airway." Presented at Department of Biology, University of Chicago, Chicago, IL, US|USA. (February 2005)

"The role of bacterial glycosidases in pneumococcal pathogenesis." Presented at The Midwest Carbohydrate Meeting, Columbus, OH, US|USA. (October 2007)

"Streptococcus pneumoniae deglycosylation: effects on colonization." Presented at Department of Microbiology, King's College, London Dental Institute, London, GB|GBR. (March 2007)

"The role of bacterial glycosidases in pneumococcal colonization." Presented at Department of Infectious Diseases and Pathology, University of Florida, Gainesville, FL, US|USA. (September 2008)

"The contribution of pneumococcal glycosidases to pathogenesis." Presented at Midwest Microbial Pathogenesis Meeting, Madison, WI, US|USA. (September 2008)

"Pneumococcal vaccines past, present, and future." Presented at 10th International Symposium on Recent Advances in Otitis Media, New Orleans, LA, US|USA. (June 2011)

"The role of pneumococcal modification of host glycoconjugates to airway colonization." Presented at Department of Microbiology, Miami University, Miami, OH, US|USA. (March 2011)

"The contribution of pneumococcal glycosidases to pathogenesis." Presented at The Ohio Branch, ASM Meeting, Athens, OH, US|USA. (April 2011)

"Pneumococcal carbohydrate transporters: food for thought." Presented at Department of Biology, Indiana University Bloomington, Bloomington, IN, US|USA. (December 2012)

"Pneumococcal carbohydrate transporters: food for thought." Presented at Institute of Microbiology and Infection, University of Birmingham, Birmingham, GB|GBR. (September 2012)

"Pneumococcal carbohydrate transporters: food for thought." Presented at Department of Laboratory Medicine, Karolinska Institutet, Stockholm, SE|SWE. (September 2012)

"The multiple contributions of pneumococcal glycosidases to pathogenesis." Presented at Ohio Agricultural Research and Development Center, Wooster, OH, US|USA. (May 2012)

"Pneumococcal carbohydrate transporters: Food for thought." Presented at Department of Biochemistry and Microbiology, Victoria, BC, CA|CAN. (October 2012)

"Mechanisms of BgaA mediated pneumococcal adherence." Presented at Europneumo, Madrid, ES|ESP. (May 2013)

"Carbohydrate-binding medules within a carbohydrate-active enzyme mediate pneumococcal adherence to host cells." Presented at FASEB, Itasca, IL, US|USA. (June 2014)

"Novel mechanisms by which pathogenic streptocci bind host carbohydrates." Presented at Department of Biological Sciences,, Dayton. (October 2016)

"Streptococcus oralis utilizes a novel mechanism of sequential adherence to host glycans." Presented at Department of Biological Sciences,, . (November 2016)

"Streptococcus oralis utilizes a novel mechanism of sequential adherence to host glycans." Presented at Department of Molecular Biology and Microbiology, Tufts University, Boston. (March 2016)

"Streptococcus oralis utilizes a novel mechanism of sequential adherence to host glycans." Presented at Department of Microbiology, University of Iowa, Iowa City. (February 2016)

 

Professional Activities

2016 - present Postdoctoral Mentorship Committee, Nicholas Wein, PhD.