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Natividad Ruiz, PhD


318 W 12th Ave
Columbus, OH 43210

Phone: (614) 292-3426

Email: ruiz.82@osu.edu

 

Current OSU Appointment

Assistant Professor, Microbiology Admin

 

Academic Advising

2010 - present Rebecca Davis The Ohio State University.
2012 - 2014 Paul Nicholson The Ohio State University.
2013 - 2014 Hildy Joseph The Ohio State University.
 

Degrees

1993 B.A., University of Kansas

1998 Ph.D., Washington University in St. Louis

 

Journal Articles

Wang,B; Ruiz,N; Pentland,A; Caparon,M. "Keratinocyte proinflammatory responses to adherent and nonadherent group A streptococci." INFECTION AND IMMUNITY. Vol. 65, no. 6. (June 1997.): 2119-2126.

Ruiz,N; Wang,B; Pentland,A; Caparon,M. "Streptolysin O and adherence synergistically modulate proinflammatory responses of keratinocytes to group A streptococci." MOLECULAR MICROBIOLOGY. Vol. 27, no. 2. (January 1998.): 337-346.

Madden,J,C; Ruiz,N; Caparon,M. "Cytolysin-mediated translocation (CMT): A functional equivalent of type III secretion in Gram-positive bacteria." CELL. Vol. 104, no. 1. (January 2001.): 143-152.

Eggert,U,S; Ruiz,N; Falcone,BV; Branstrom,AA; Goldman,RC; Silhavy,TJ; Kahne,D. "Genetic basis for activity differences between vancomycin and glycolipid derivatives of vancomycin." SCIENCE. Vol. 294, no. 5541. (October 2001.): 361-364.

Ruiz,N; Peterson,C,N; Silhavy,TJ. "RpoS-dependent transcriptional control of sprE: Regulatory feedback loop." JOURNAL OF BACTERIOLOGY. Vol. 183, no. 20. (October 2001.): 5974-5981.

Ruiz,N; Silhavy,TJ. "Constitutive activation of the Escherichia coli Pho regulon upregulates [begin-italics]rpoS[end-italics] translation in an Hfq-dependent fashion." JOURNAL OF BACTERIOLOGY. Vol. 185, no. 20. (October 2003.): 5984-5992.

Peterson,C,N; Ruiz,N; Silhavy,TJ. "RpoS proteolysis is regulated by a mechanism that does not require the SprE (RssB) response regulator phosphorylation site." JOURNAL OF BACTERIOLOGY. Vol. 186, no. 21. (November 2004.): 7403-7410.

Wu,T; Malinverni,J; Ruiz,N; Kim,S; Silhavy,T,J; Kahne,D. "Identification of a multicomponent complex required for outer membrane biogenesis in Escherichia coli." CELL. Vol. 121, no. 2. (April 2005.): 235-245.

Ruiz,N; Silhavy,TJ. "Sensing external stress: watchdogs of the Escherichia coli cell envelope." CURRENT OPINION IN MICROBIOLOGY. Vol. 8, no. 2. (April 2005.): 122-126.

Ruiz,N; Falcone,B; Kahne,D; Silhavy,T,J. "Chemical conditionality: A genetic strategy to probe organelle assembly." CELL. Vol. 121, no. 2. (April 2005.): 307-317.

Ruiz,N; Wu,T; Kahne,D; Silhavy,TJ. "Probing the barrier function of the outer membrane with chemical conditionality." ACS CHEMICAL BIOLOGY. Vol. 1, no. 6. (January 2006.): 385-395.

Ruiz,N; Kahne,D; Silhavy,TJ. "Advances in understanding bacterial outer-membrane biogenesis." NATURE REVIEWS MICROBIOLOGY. Vol. 4, no. 1. (January 2006.): 57-66.

Button,JE; Silhavy,TJ; Ruiz,N. "A suppressor of cell death caused by the loss of sigma(E) downregulates extracytoplasmic stress responses and outer membrane vesicle production in Escherichia coli." JOURNAL OF BACTERIOLOGY. Vol. 189, no. 5. (March 2007.): 1523-1530.

Ruiz,N; Gronenberg,LS; Kahne,D; Silhavy,TJ. "Identification of two inner-membrane proteins required for the transport of lipopolysaccharide to the outer membrane of Escherichia coli." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. Vol. 105, no. 14. (April 2008.): 5537-5542.

Ruiz,N. "Bioinformatics identification of MurJ (MviN) as the peptidoglycan lipid II flippase in Escherichia coli." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. Vol. 105, no. 40. (October 2008.): 15553-15557.

Vertommen,D*; Ruiz,N*; Leverrier,P; Silhavy,TJ; Collet,JF. "Characterization of the role of the Escherichia coli periplasmic chaperone SurA using differential proteomics." PROTEOMICS. Vol. 9, no. 9. (May 2009.): 2432-2443.

Ruiz,N; Kahne,D; Silhavy,TJ. "TIMELINE Transport of lipopolysaccharide across the cell envelope: the long road of discovery." NATURE REVIEWS MICROBIOLOGY. Vol. 7, no. 9. (September 2009.): 677-683.

Ruiz,N. "Streptococcus pyogenes YtgP (Spy_0390) complements Escherichia coli strains depleted of the putative peptidoglycan flippase MurJ." ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. Vol. 53, no. 8. (August 2009.): 3604-3605.

Chng,SS; Ruiz,N; Chimalakonda,G; Silhavy,TJ; Kahne,D. "Characterization of the two-protein complex in Escherichia coli responsible for lipopolysaccharide assembly at the outer membrane." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. Vol. 107, no. 12. (March 2010.): 5363-5368.

Ruiz,N; Chng,SS; Hiniker,A; Kahne,D; Silhavy,TJ. "Nonconsecutive disulfide bond formation in an essential integral outer membrane protein." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. Vol. 107, no. 27. (July 2010.): 12245-12250.

Chimalakonda G; Ruiz N; Chng SS; Garner RA; Kahne D; Silhavy TJ. "Lipoprotein LptE is required for the assembly of LptD by the beta-barrel assembly machine in the outer membrane of Escherichia coli.." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. Vol. 108, no. 6. (February 2011.): 2492-.

Karoamoto, M., Cline, S., Redding, K., Ruiz, N., and Hamel, P. P.. "Lumen Thiol Oxidoreductase1, a disulfide bond-forming catalyst, is required for the assembly of photosystem II in Arabidopsis." PLANT CELL. Vol. 23, no. 12. (January 2012.): 4446-4461.

Freinkman,E; Okuda,S; Ruiz,N; Kahne,D. "Regulated assembly of the transenvelope protein complex required for lipopolysaccharide export." BIOCHEMISTRY. Vol. 51, no. 24. (June 2012.): 4800-4806.

Yao,Z; Davis,RM; Kishony,R; Kahne,D; Ruiz,N. "Regulation of cell size in response to nutrient availability by fatty acid biosynthesis in Escherichia coli." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. Vol. 109, no. 38. (September 2012.): E2561-E2568.

Ruiz,N. "A bird's eye view of the bacterial landscape.." Methods in molecular biology (Clifton, N.J.). Vol. 966, (January 2013.): 1-14.

Butler,EK; Davis,RM; Bari,V; Nicholson,PA; Ruiz,N. "Structure-Function Analysis of MurJ Reveals a Solvent-Exposed Cavity Containing Residues Essential for Peptidoglycan Biogenesis in Escherichia coli.." JOURNAL OF BACTERIOLOGY. Vol. 195, no. 20. (October 2013.): 4639-4649.

Sherman DJ, Lazarus MB, Murphy L, Liu C, Walker S, Ruiz N*, Kahne D*.. "Decoupling catalytic activity from biological function of the ATPase that powers lipopolysaccharide transport." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. Vol. 111, no. 3. (March 2014.): 4982-4987.

Nicolaes,V; El Hajjaji,H; Davis,RM; Van der Henst,C; Depuydt,M; Leverrier,P; Aertsen,A; Haufroid,V; Ollagnier de Choudens,S; De Bolle,X; Ruiz,N; Collet,JF. "Insights into the function of YciM, a heat shock membrane protein required to maintain envelope integrity in Escherichia coli.." JOURNAL OF BACTERIOLOGY. Vol. 196, no. 2. (January 2014.): 300-309.

 

Presentations

"Understanding envelope biogenesis in E. coli." Presented at Center for Microbial Pathogenesis, Columbus, OH. (March 2011)

"Understanding LPS biogenesis in Escherichia coli." Presented at Ohio Branch of the American Society for Microbiology Annual Meeting, Athens, OH. (April 2011)

"Understanding the role on MurJ in peptidoglycan biogenesis." Presented at Center for Microbial Interface Biology, Columbus. (February 2011)

"Understanding LPS transport in Escherichia coli." Presented at 2011 ASM General Meeting, New Orleans, LA. (May 2011)

"Understanding envelope biogenesis in Escherichia coli." Presented at Department of Biochemistry, Columbus, OH. (May 2011)

"Biogenesis of the Bacterial Cell Surface Symposium." Presented at ASM General Meeting, New Orleans. (May 2011)

"Understanding envelope biogenesis in E. coli." Presented at Novartis Institutes for Biomedical Research, Emeryville, CA. (September 2011)

"Understanding LPS transport in Escherichia coli." Presented at Midwest Microbial Pathogenesis Conference, Ann Arbor. (October 2011)

"Understanding envelope biogenesis in E. coli." Presented at Department of Biology, Microbiology Seminar Series, Bloomington, IN. (March 2012)

"Understanding envelope biogenesis in E. coli." Presented at Department of Microbiology, Oxford. (February 2012)

"Envelope biogenesis in E. coli." Presented at Cold Spring Harbor Laboratory Advanced Bacterial Genetics Course, Cold Spring Harbor, NY. (June 2012)

"Regulation of cell size in response to nutrient availability by fatty acid biosynthesis in Escherichia coli." Presented at Bacterial Cell Surfaces Gordon Conference, West Dover, VT. (June 2012)

"Understanding MurJ function through a SCAM." Presented at Genetic Approaches to Understanding Complex Cellular Processes Conference, Sedona, AZ. (March 2013)

"Structure-function analysis of MurJ." Presented at Department of Microbiology, South San Francisco, CA. (July 2013)

"Life Beyond the Cytosol." Presented at 2013 Molecular Genetics of Bacteria and Phages Meeting, Madison, WI. (August 2013)

"Structure-function analysis of MurJ." Presented at Department of Microbiology, Urbana, IL. (October 2013)

"Structure-function analysis of MurJ." Presented at 2013 Molecular Genetics of Bacteria and Phages Meeting, Madison, WI. (August 2013)

"Structure-function analysis of MurJ." Presented at XII Pan-American Biochemistry and Molecular Biology Congress, Puerto Varas, CL|CHL. (November 2013)

"Structure-function analysis of MurJ." Presented at Department of Biological Sciences, Edmonton, CA|CAN. (November 2013)

"Envelope biogenesis in Escherichia coli." Presented at Department of Microbiology, Santiago de Chile, CL|CHL. (November 2013)

"Regulation of envelope growth in Escherichia coli." Presented at The Child Health Research Center Speaker Series, Columbus, OH. (January 2013)

"Envelope biogenesis in Escherichia coli: Who is flipping lipid II?." Presented at OSU Chemistry-Biology Interface Training Program, Columbus, OH. (February 2013)

"Envelope biogenesis in E. coli." Presented at Department of Biology Seminar Series, Gambier, OH. (February 2013)

"Transport of peptidoglycan precursors across membranes." Presented at Protein Transport Across Cell Membranes Gordon Conference, Galveston, TX. (March 2014)

"Understanding the function of MurJ in bacterial cell wall biogenesis." Presented at Department of Molecular Genetics, Columbus, OH. (March 2014)

 

Unpublished Works

1. Sham, L. T., Butler, EK, Lebar, MD, Kahne, D, Bernhardt, TG*, and Ruiz, N*. MurJ is the flippase of lipid-linked precursors for peptidoglycan biogenesis. April 2014.

Malojčić, G, Andres, D, Grabowicz, M, Ruiz, N, Silhavy, TJ, Kahne, D. LptE binds to and alters the physical state of LPS to catalyze its assembly at the cell surface. February 2014.