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Eugene Leys, PhD


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305 W Twelfth Avenue
Columbus, OH 43210

Phone: (614) 292-5273

Email: leys.1@osu.edu

 

Current OSU Appointment

Professor, Biosciences

 

Advising Narrative

Health Professions - Laboratory/Cellular

Chapters in Books

Sprading AC and Leys EJ. "Slow Replication Fork Movement During Drosophila Chorion Gene Amplification." In Eukaryotic DNA Replication. 305-309. Cold Spring Harbor, NY, US|USA: Cold Spring Harbor Press, January 1989.

Eugene Leys, Ann Griffen, Purnima Kumar, Mark Maiden. "Isolation, Classification, and Identification or Oral Microorganisms." In Oral Microbiology and Immunology. first ed. Edited by Lamont, Burne, Lantz, LeBlanc. 73-88. Washington, US|USA: ASM Press, January 2006.

 

Degrees

1978 B.S., University Of Arizona

1983 Ph.D., Baylor College of Medicine

 

Editorial Activities

present Microbiology
present Journal of Clinical Microbiology
present European Journal of Oral Sciences
present FEMS Microbiology
present BMC Genomics
present BMC Microbiology
present Journal of Dental Research
present Journal of Bacteriology
present Infection and Immunity
present Genetics
present Clinical and Vaccine Immunology
present Biotechniques
present Applied and Environmental Microbiology
2011 - present Molecular Oral Microbiology
 

Journal Articles

Leys EJ, Crouse GF, Kellems RE. "Dihydrofolate reductase gene expression in cultured mouse cells is regulated by transcript stabilization in the nucleus." Journal of Cell Biolgoy. Vol. 1, no. 99. (January 0.): 180-187.

Leys EJ, Kellems RE. "Control of dihydrofolate reductase messenger ribonucleic acid production.." Molecular and Cellular Biology. Vol. 11, no. 1. (November 1981.): 961-971.

Yoder SS, Robberson BL, Leys EJ, Hook AG, Al-Ubaidi M, Yeung CY, Kellems RE, Berget SM.. "Control of cellular gene expression during adenovirus infection: induction and shut-off of dihydrofolate reductase gene expression by adenovirus type 2.." Molecular and Cellular Biology. Vol. 5, no. 3. (May 1983.): 819-828.

Frayne EG, Leys EJ, Crouse GF, Hook AG, Kellems RE. "Transcription of the mouse dihydrofolate reductase gene proceeds unabated through seven polyadenylation sites and terminates near a region of repeated DNA." Molecular and Cellular Biology. Vol. 12, no. 4. (December 1984.): 2921-2914.

Crouse GF, Leys EJ, McEwan RN, Frayne EG, Kellems RE.. "Analysis of the mouse dhfr promoter region: existence of a divergently transcribed gene." Molecular and Cellular Biology. Vol. 8, no. 5. (August 1985.): 1847-1858.

Griffen AL, Leys EJ, Fuerst PA.. "Strain identification of Actinobacillus actinomycetemcomitans using the polymerase chain reaction." Oral Microbiology and Immunology. Vol. 4, no. 7. (August 1992.): 240-243.

Leys EJ, Griffen AL, Strong SJ, Fuerst PA. "Detection and strain identification of Actinobacillus actinomycetemcomitans by nested PCR." Journal of Clinical Microbiology. Vol. 5, no. 32. (May 1994.): 1288-1294.

McClellan DL, Griffen AL, Leys EJ.. "Age and prevalence of Porphyromonas gingivalis in children." Journal of Clinical Microbiology. Vol. 8, no. 34. (August 1996.): 2017-2019.

Tuite-McDonnell M, Griffen AL, Moeschberger ML, Dalton RE, Fuerst PA, Leys EJ. "Concordance of Porphyromonas gingivalis colonization in families." Journal of Clinical Microbiology. Vol. 2, no. 35. (February 1997.): 455-461.

Griffen AL, Becker MR, Lyons SR, Moeschberger ML, Leys EJ. "Prevalence of Porphyromonas gingivalis and periodontal health status." Journal of Clinical Microbiology. Vol. 11, no. 36. (November 1998.): 3239-3242.

Leys EJ, Smith JH, Lyons SR, Griffen AL. "Identification of Porphyromonas gingivalis strains by heteroduplex analysis and detection of multiple strains." Journal of Clinical Microbiology. Vol. 12, no. 37. (December 1999.): 3906-3911.

Rumpf RW, Griffen AL, Wen BG, Leys EJ. "Sequencing of the ribosomal intergenic spacer region for strain identification of Porphyromonas gingivalis." Journal of Clinical Microbiology. Vol. 8, no. 37. (August 1999.): 2723-2725.

Griffen AL, Lyons SR, Becker MR, Moeschberger ML, Leys EJ.. "Porphyromonas gingivalis strain variability and periodontitis." Journal of Clinical Microbiology. Vol. 12, no. 37. (December 1999.): 4028-4033.

Rumpf RW, Griffen AL, Leys EJ. "Phylogeny of Porphyromonas gingivalis by ribosomal intergenic spacer region analysis." Journal of Clinical Microbiology. Vol. 5, no. 38. (May 2000.): 1807-1810.

Lyons SR, Griffen AL, Leys EJ. "Quantitative real-time PCR for Porphyromonas gingivalis and total bacteria." Journal of Clinical Microbiology. Vol. 6, no. 38. (June 2000.): 2362-2365.

Lamell CW, Griffen AL, McClellan DL, Leys EJ. "Acquisition and colonization stability of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in children." Journal of Clinical Microbiology. Vol. 3, no. 38. (March 2000.): 1196-1199.

Becker MR, Paster BJ, Leys EJ, Moeschberger ML, Kenyon SG, Galvin JL, Boches SK, Dewhirst FE, Griffen AL.. "Molecular analysis of bacterial species associated with childhood caries.." Journal of Clinical Microbiology. Vol. 3, no. 40. (March 2002.): 1001-1009.

Leys EJ, Lyons SR, Moeschberger ML, Rumpf RW, Griffen AL.. "Association of Bacteroides forsythus and a novel Bacteroides phylotype with periodontitis.." Journal of Clinical Microbiology. Vol. 3, no. 40. (March 2002.): 821-825.

Kumar PS, Griffen AL, Barton JA, Paster BJ, Moeschberger ML, Leys EJ. "New bacterial species associated with chronic periodontitis.." Journal of Dental Research. Vol. 5, no. 82. (May 2003.): 338-344.

Kumar PS, Griffen AL, Moeschberger ML, Leys EJ. "Identification of candidate periodontal pathogens and beneficial species by quantitative 16S clonal analysis.." Journal of Clinical Microbiology. Vol. 8, no. 43. (August 2005.): 3944-3955.

Kumar PS, Leys EJ, Bryk JM, Martinez FJ, Moeschberger ML, Griffen AL. "Changes in periodontal health status are associated with bacterial community shifts as assessed by quantitative 16S cloning and sequencing.." Journal of Clinical Microbiology. Vol. 10, no. 44. (October 2006.): 3665-3673.

Aas JA, Griffen AL, Dardis SR, Lee AM, Olsen I, Dewhirst FE, Leys EJ, Paster BJ.. "Bacteria of dental caries in primary and permanent teeth in children and young adults.." Journal of Clinical Microbiology. Vol. 4, no. 46. (April 2008.): 1407-1417.

Igboin CO, Griffen AL, Leys EJ.. "Porphyromonas gingivalis strain diversity.." Journal of Clinical Microbiology. Vol. 10, no. 47. (October 2009.): 3073-3081.

Gross EL, Leys EJ, Gasparovich SR, Firestone ND, Schwartzbaum JA, Janies DA, Asnani K, Griffen AL.. "Bacterial 16S sequence analysis of severe caries in young permanent teeth.." Journal of Clinical Microbiology. Vol. 11, no. 48. (November 2010.): 4121-4126.

Igboin CO, Tordoff KP, Moeschberger ML, Griffen AL, Leys EJ.. "Porphyromonas gingivalis-host interactions in a Drosophila melanogaster model.." Infection and Immunity. Vol. 1, no. 79. (January 2011.): 449-458.

Igboin CO, Moeschberger ML, Griffen AL, Leys EJ.. "Porphyromonas gingivalis virulence in a Drosophila melanogaster model.." Infection and Immunity. Vol. 1, no. 79. (January 2011.): 439-448.

Catherine E. Moffatt1, Sarah E. Whitmore1, Ann L. Griffen2, Eugene J. Leys3, Richard J. Lamont1. "Filifactor alocis interactions with gingival epithelial cells." Molecular Oral Microbiology. (January 2011.): -.

Griffen AL, Beall CJ, Firestone ND, Gross EL, Difranco JM, Hardman JH, Vriesendorp B, Faust RA, Janies DA, Leys EJ.. "CORE: a phylogenetically-curated 16S rDNA database of the core oral microbiome.." PLoS One. Vol. 4, no. 6. (April 2011.): e19051-.

 

Reference Works

January 2002 E.J. LEYS, C. IGBOIN, and A.L. GRIFFEN."Bacteroides forsythus strain variability in periodontitis" . : 0738
January 2002 J.A. BARTON1, P. KUMAR1, B.J. PASTER2, A.L. GRIFFEN1, and E.J. LEYS."New bacterial species associated with chronic periodontitis" . : 2926
January 2003 J.A. BARTON1, P.S. KUMAR2, A.L. GRIFFEN1, B.J. PASTER3, and E.J. LEYS2."New Candidate Bacterial Pathogens in Chronic Periodontitis" . : 1738
January 2003 J.A. AAS1, S.R. DARDIS2, A.L. GRIFFEN3, L.N. STOKES1, A.M. LEE1, N.A. PRINCE1, I. OLSEN4, F.E. DEWHIRST1, E.J. LEYS2, and B.J. PASTE."Molecular Analysis of Bacteria Associated with Caries in Permanent Teeth" . : 1025
January 2003 J. BRYK1, F.J. MARTINEZ1, E.J. LEYS2, and A.L. GRIFFEN1."Stability of Porphyromonas gingivalis Colonization in the Gingival Sulcus" . : 0372
January 2003 P.S. KUMAR1, A.L. GRIFFEN2, M.R. ZIANNI1, P.M. PRESHAW3, and E.J. LEYS."Quantitative Identification of the Bacteria in Chronic Periodontitis by Clonal Analysis" . : 1737
January 2004 E.L. MCILVAINE, Z. WANG, P.S. KUMAR, A.L. GRIFFEN, and E.J. LEY."New candidate bacterial pathogens in chronic periodontitis II" . : 1038
January 2004 P.S. KUMAR, A.L. GRIFFEN, J. BRYK, F.J. MARTINEZ, and E.J. LEYS."Colonization Stability of Subgingival Bacteria by Clonal Analysis" . : 4063
January 2004 J.A. AAS1, S. DARDIS2, A.L. GRIFFEN2, L. STOKES3, A.M. LEE3, I. OLSEN4, F.E. DEWHIRST3, E.J. LEYS2, and B.J. PASTER."Dental caries; an old disease with largely unknown microbiota" . : 0221
January 2004 J.A. AAS1, L.C. GERSTMANN2, S.M. BARBUTO1, A.L. GRIFFEN2, E.J. LEYS2, I. OLSEN3, F.E. DEWHIRST1, and B.J. PASTER."Molecular Analysis of Bacteria Associated with Incipient Early Childhood Caries" . : 3430
January 2004 J. BRYK, F.J. MARTINEZ, E.J. LEYS, and A.L. GRIFFEN,."2-Year Stability of Bacteria in the Gingival Sulcus" . : 3660
January 2004 B.P.F.A. GOMES1, A.L. GRIFFEN2, P.S. KUMAR2, E.T. PINHEIRO1, R.C. JACINTO1, E.L. SOUSA1, C.C.R. FERRAZ1, A.A. ZAIA1, F.J. SOUZA-FILHO1, and E.J. LEYS2,."Culture and PCR analysis of bacteria in infected root canals" . : 0062
January 2004 B.P.F.A. GOMES1, A.L. GRIFFEN2, P.S. KUMAR2, E.T. PINHEIRO1, E.L.R. SOUSA1, R.C. JACINTO1, C.C.R. FERRAZ1, A.A. ZAIA1, F.J. SOUZA-FILHO1, and E.J. LEYS."Molecular and culture analysis of bacteria in infected root canals" . : 1040
January 2005 D.B. CLAMAN, E.J. LEYS, and A.L. GRIFFEN."Identification of Red-Fluorescing Caries-Associated Bacteria" . : 3461
January 2005 P.S. KUMAR1, A.L. GRIFFEN1, J.M. BRYK2, F.J. MARTINEZ2, and E.J. LEYS."Association between subgingival microbial stability and periodontal status" . : 2257
January 2005 J.M. BRYK, F.J. MARTINEZ, E.J. LEYS, and A.L. GRIFFEN."3-Year Stability of Bacteria in the Gingival Sulcus" . : 0999
January 2005 J.A. AAS1, S.R. DARDIS2, A.L. GRIFFEN2, L.N. STOKES3, A.M. LEE3, I. OLSEN4, F.E. DEWHIRST3, E.J. LEYS2, and B.J. PASTER."Most of the Caries Microbiota Has Not Yet Been Cultivated" . : 2805
January 2005 A.L. BEROSKE, B. LEBLEBICIOGLU, A.L. GRIFFEN,."Transmission of Peptostreptococcaceae within Families" . : 3487
January 2006 Igboin, Leys, Griffen."Comparison of genome content and virulence of Porphyromonas gingivalis strains" .
January 2006 A.L. BEROSKE, A.L. GRIFFEN, and E.J. LEYS."Acquisition by Children of Gram-Positive Bacteria Associated with Periodontitis" . : 1102
January 2007 K.M. ASNANI1, E.L. GROSS1, B.J. PASTER2, E.J. LEYS1, and A.L. GRIFFEN."Molecular analysis of microbial diversity in caries and health" . : 2285
January 2007 E.L. GROSS, S.R. KUTSCH, K.M. ASNANI, E.J. LEYS, and A.L. GRIFFEN,."Molecular Analysis of the Biofilm in Incipient Early Childhood Caries" . : 2286
January 2007 A.L. BEROSKE, A.L. GRIFFEN, and E.J. LEY."Stability of Bacteria Associated with Periodontitis in Children" . : 1171
January 2008 E.L. GROSS, K.M. ASNANI, E.J. LEYS, D. JANIES, J.A. SCHWARTZBAUM, S. GASPAROVICH, and A.L. GRIFFEN."Molecular Microbiological Analysis of Severe Caries in Young Permanent Teeth" . : 0312
January 2008 E.L. GROSS, A.L. GRIFFEN, and E.J. LEYS."Optimizing Bacterial DNA Isolation from Oral Samples" . : 2118
January 2008 A.L. BEROSKE, A.L. GRIFFEN, and E.J. LEYS."Concordance of Treponema denticola Colonization in Families" . : 0310
January 2008 C.H. SISSONS1, L. WONG1, E.J. LEYS2, A.L. GRIFFEN2, and S.K. FILOCHE."Generation and Characterization of Microcosm Biofilms Modelling Pathogen-Specific Plaques" . : 0157
January 2009 E. MOKRZAN, R. FISHMAN, M.H. WELLS, E. LEYS, and A. GRIFFEN."Molecular analysis of incipient caries biofilm in young permanent teeth" . : 3416
January 2009 C. IGBOIN, K. TORDOFF, M. MOESCHBERGER, A. GRIFFEN, and E. LEYS."Interaction of the Porphyromonas gingivalis capsule with infected Drosophila melanogaster" . : 2150
January 2011 T. PREMARAJ, C. IGBOIN, A.L. GRIFFEN, and E.J. LEYS."Quantitation of periodontal pathogens by real time PCR" . : 3666
January 2011 E.L. GROSS1, E.J. LEYS1, B. VRIESENDORP1, N.D. FIRESTONE1, D.A. JANIES1, J.S. MARTIN1, B.J. PASTER2, and A.L. GRIFFEN."Molecular Microbiological Analysis of Severe Caries in Primary Teeth" . : 774
January 2011 E. LEYS1, C. BEALL1, N. FIRESTONE1, J. DIFRANCO1, P. KUMAR2, J. CAMPBELL3, M. PODAR3, and A. GRIFFEN1,."Periodontitis-associated Bacterial Community Shifts Analyzed by 16S Pyrosequencing" . : 1641
January 2011 E. GROSS, E. LEYS, C. BEALL, and A. GRIFFEN."The Relationship of Streptococcus mutans and Lactobacillus in Childhood Caries" . : 2773
January 2011 A. GRIFFEN1, C. BEALL1, N. FIRESTONE1, E. GROSS2, J. DIFRANCO1, J. HARDMAN3, B. VRIESENDORP4, D. JANIES3, and E. LEYS1."CORE: A Phylogenetically-Curated 16S rDNA Database of the Oral Microbiome" . : 443
January 2011 C. BEALL, A. GRIFFEN, and E. LEYS."Examination of the Oral Microbiome by Illumina Deep Sequencing" . : 418

Presentations

"A molecular View of Oral Bacteria in Health and Disease." Cleveland, OH, US|USA. (June 2004)

"Epidemiology of P. gingivalis." Presented at Mark Wilson Conference on Oral Infectious Diseases, Naples, FL, US|USA. (February 2007)

"Molecular and Population Biology of Periodontal Pathogens." Dunedin, NZ|NZL. (April 2007)

"A new system for studying bacterial-host interactions.." Presented at Mark Wilson Conference on Oral Infectious Diseases, Cancun, MX|MEX. (February 2008)

"What Phylogenetics Tells Us About the Composition of the Oral Microbiome." Presented at Mark Wilson conference on oral infectious diseases., . (February 2009)

"16S deep sequencing and Periodontitis." Presented at Mark Wilson Conference on oral infectious diseases, Cancun, MX|MEX. (February 2010)

"Molecular Identification of Periodontal Pathogens." Newark, NJ, US|USA. (May 2010)

"Metagenomics of human oral bacteria." Presented at 454 Genome Sequencing Seminars, Columbus, OH, US|USA. (May 2011)

"Bacteria-host interactions in a Drosophila model." Presented at Mark Wilson conference on Oral Infectious diseases, San Juan, Pueto Rico, US|USA. (February 2011)

 

Professional Activities

2002 - present Grant review panel. NIH. Bethesda, MD.
2003 - present National Board Review. Chicago, IL.