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Emilio Flano, PhD


Staff photo

700 Childrens Dr
Ctr for Vaccines & Immunity
Columbus, OH 43205

Phone: (614) 722-4064

Email: flano.1@osu.edu

 

Current OSU Appointment

Associate Professor, SBS-Microbial Infectn/Immunity

 

Biographical

My major interest is the study of the immune response to viral infections. We use a variety of immunological, cellular and molecular techniques, different viruses and animal models to approach these studies. I started working in viral immunology in 1997, during my postdoctoral training in the Department of Immunology at St. Jude’s Children’s Hospital, Memphis TN under the supervision of Dr. P.C. Doherty (1996 Nobel laureate in Medicine). My work focused on the immunology of viral infections, specifically of γ-herpesviruses. In 2000, I joined the Trudeau Institute, Saranac Lake, NY as a Research Associate and in 2001 I was promoted to Research Assistant Member. At the Trudeau Institute I continued my studies on immunity to γ-herpesviruses under the supervision of Dr. M.A. Blackman. During my training, I became a highly productive scientist and a well-know researcher in the field of viral immunology. My studies were published in prestigious peer-reviewed journals such as The Journal of Immunology, The Journal of Virology and The Journal of Experimental Medicine. During this time, I was awarded a Keystone Symposia Scholarship (2000) and a Junior Faculty Award (2002) by the American Association of Immunologists. In 2004 I joined The Research Institute at Nationwide Children’s Hospital and The Ohio State University as an Assistant Professor. My research program focuses on the study of the immune response to chronic and respiratory viral infections.

Academic Advising

2012 - present Rebecca Ortiz The Research Institute at Nationwide Childrens Hospital. Graduated 2011.
 

Editorial Activities

present J. Gen. Virol.
2011 - present ISRN Immunology
 

Journal Articles

Alvarez,F; Flano,E; Villena,A,J; Zapata,A; Razquin,B,E. "SEASONAL INTRATHYMIC ERYTHROPOIETIC ACTIVITY IN TROUT." DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY. Vol. 18, no. 5. (September 1994.): 409-420.

Flaño, E., López-Fierro, P., Razquin, B.E., Villena, A.. "In vitro differentiation of eosinophilic granular cells in Renibacterium salmoninarum-infected gill cultures from rainbow trout.." Fish Shellfish Immunol. Vol. 6, (January 1996.): 173-184.

Flaño, E., Kaattari, S.L., Razquin, B., Villena, A.. "Histopathology of the thymus of coho salmon, Oncorhynchus kisutch, experimentally infected with Renibacterium salmoninarum.." Diseases Aquatic Organisms. Vol. 26, (January 1996.): 11-18.

Alvarez, F., Flaño, E., Castillo, A., López-Fierro, P., Razquin, B., Villena, A.. "Tissue distribution of barrier cells in the hematopoietic and lymphoid organs of salmonids.." Anatomical Record. Vol. 245, (January 1996.): 17-24.

Flano,E; Alvarez,F; LOPEZFIERRO,P; Razquin,B,E; Villena,A,J; Zapata,A,G. "In vitro and in situ characterization of fish thymic nurse cells." DEVELOPMENTAL IMMUNOLOGY. Vol. 5, no. 1. (January 1996.): 17-24.

Flaño, E., López-Fierro, P., Razquin, B., Kaattari, S.L., Villena, A.. "Histopathology of the renal and splenic haemopoietic tissues of coho salmon, Oncorhynchus kisutch, experimentally infected with Renibacterium salmoninarum.." Diseases Aquatic Organisms. Vol. 24, (January 1996.): 107-115.

McIntosh,D; Flano,E; Grayson,T,H; Gilpin,M,L; Austin,B; Villena,A,J. "Production of putative virulence factors by Renibacterium salmoninarum grown in cell culture." MICROBIOLOGY-SGM. Vol. 143, (October 1997.): 3349-3356.

Flano,E; LOPEZFIERRO,P; Razquin,B,E; Villena,A. "In vitro proliferation of eosinophilic granular cells in gill cultures from rainbow trout." FISH & SHELLFISH IMMUNOLOGY. Vol. 7, no. 7. (October 1997.): 519-521.

Flano,E; Lopez-Fierro,P; Alvarez,F; Razquin,B; Villena,A. "Splenic cultures from rainbow trout, Oncorhynchus mykiss: establishment and characterisation." FISH & SHELLFISH IMMUNOLOGY. Vol. 8, no. 8. (November 1998.): 589-606.

Schroder,M,B; Flano,E; PILSTROM,L; Jorgensen,T,O. "Localisation of Ig heavy chain mRNA positive cells in Atlantic cod (Gadus morhua L) tissues identified by in situ hybridisation." FISH & SHELLFISH IMMUNOLOGY. Vol. 8, no. 8. (November 1998.): 565-576.

Hernanz Moral, C., Flaño del Castillo E., Lopez Fierro, P., Villena Cortes, A., Anguita Castillo, J., Cascon Soriano, A., Sanchez Salazar, M., Razquin Peralta, B., Naharro Carrasco, G.. "Molecular characterization of the Aeromonas hydrophila aroA gene and potential use of an auxothrophic aroA mutant as live attenuated vaccine.." Infect Immunity. Vol. 66, (January 1998.): 1813-1821.

Coppola, M.A., Flaño, E., Nguyen, P., Hardy, C.L., Cardin, R.D., Shastri, N., Woodland, D.L., Blackman, M.A.. "MHC-independent stimulation of CD8+ T cells in vivo during latent murine γ-herpesvirus infection.." J Immunol. Vol. 163, (January 1999.): 1481-1489.

Liu, L., Flaño, E., Usherwood, E.J., Surman, S.L., Blackman, M.A., Woodland, D.L.. "Lytic cycle T cell epitopes are expressed in two distinct phases during MHV-68 infection.." J Immunol. Vol. 163, (January 1999.): 868-874.

Flaño, E., Woodland, L.D., Blackman, M.A.. "Requirement for CD4+T cells in Vb4+CD8+ T cell activation associated with latent murine gammaherpesvirus infection.." J Immunol. Vol. 163, (January 1999.): 3403-3408.

Flaño, E., Husain, S.M., Sample, J.T., Woodland, D.L., Blackman, M.A.. "Latent murine γ-herpesvirus infection is established in activated B cells, dendritic cells and macrophages.." J Immunol. Vol. 165, (January 2000.): 1074-1081.

Blackman,M,A; Flano,E; Usherwood,E; Woodland,D,L. "Murine gamma-herpesvirus-68: a mouse model for infectious mononucleosis?." MOLECULAR MEDICINE TODAY. Vol. 6, no. 12. (December 2000.): 488-490.

McIntosh,D; Austin,B; Flano,E; Villena,A; Martinez-Pereda,J,A; Tarazona,J,V. "Lack of uptake of Renibacterium salmoninarum by gill epithelia of rainbow trout." JOURNAL OF FISH BIOLOGY. Vol. 56, no. 5. (May 2000.): 1053-1061.

Woodland, D. L., Flaño, E., Usherwood, E. J., Liu, L., Kim, I.-J., Husain, S. M., Sample, J. T., and Blackman, M. A.. "Antigen expression during murine γ -herpesvirus infection.." Immunobiology. Vol. 204, (January 2001.): 649-658.

Hardy, C.L., Flaño, E., Cardin, R.C., Kim, I.-J., Nguyen, P., King, S., Woodland, D.L., Blackman, M.A.. "Factors controlling CD8+ T cell activation associated with murine γ-herpesvirus-induced infectious mononucleosis.." Viral Immunol. Vol. 14, (January 2001.): 391-402.

Flano,E; Woodland,D,L; Blackman,M,A; Doherty,P,C. "Analysis of virus-specific CD4(+) T cells during long-term gammaherpesvirus infection." JOURNAL OF VIROLOGY. Vol. 75, no. 16. (August 2001.): 7744-7748.

Woodland, D.L., Usherwood, E.J., Liu, L., Flaño, E., Kim, I.-J., Blackman, M.A.. "Vaccination against murine γ -herpesvirus infection.." Viral Immunol. Vol. 14, (January 2001.): 217-226.

Flaño, E, Woodland, D.L., Blackman, M.A.. "A mouse model for infectious mononucleosis.." Immunologic Research. Vol. 24, (January 2001.): 353-369.

Flaño, E., Kim, I.J., Woodland, D.L., and Blackman, M.A.. "γ -Herpesvirus latency is preferentially maintained in splenic germinal center and memory B cells.." J Exp Med. Vol. 196, (January 2002.): 1363-1372.

Blackman,M,A; Flano,E. "Persistent gamma-herpesvirus infections: What can we learn from an experimental mouse model?." JOURNAL OF EXPERIMENTAL MEDICINE. Vol. 195, no. 7. (April 2002.): F29-F32.

Kim, I.-J., Flaño, E., Woodland, D.L., Blackman, M.A.. "Antibody-mediated control of persistent γ -herpesvirus infection.." J Immunol. Vol. 168, (January 2002.): 3958-3964.

Flaño, E., Kim, I-J., Woodland, D.L. and Blackman, M.A.. "Differential γ -herpesvirus distribution in distinct anatomical locations and cell subsets during persistent infection in mice.." J Immunol. Vol. 170, (January 2003.): 3828-3834.

Macrae, A.I., Usherwood, E.J., Husain, S.M., Flaño, E., Kim, I.J., Woodland, D.L., Nash, A.A., Blackman, M.A., Sample, J.T. and Stewart, J.P.. "MHV-68 M2 protein is a B cell-associated antigen important for latency but not lymphocytosis.." J Virol. Vol. 77, (January 2003.): 9700-9709.

Kim,I,J; Flano,E; Woodland,D,L; Lund,F,E; Randall,T,D; Blackman,M,A. "Maintenance of long term gamma-herpesvirus B cell latency is dependent on CD40-mediated development of memory B cells." JOURNAL OF IMMUNOLOGY. Vol. 171, no. 2. (July 2003.): 886-892.

Flaño, E., Hardy, C.L., Kim, I.J., Frankling, C., Coppola, M.A., Nguyen, P., Woodland, D.L., and Blackman, M.A.. "T cell reactivity during infectious mononucleosis and latent stages of γ-herpesvirus infection in mice.." J Immunol. Vol. 172, (January 2004.): 3078-3085.

Flaño, E., Jia, Q., Moore, J., Woodland, D.L., Sun, R. and Blackman, M.A.. "Early establishment of gamma-herpesvirus latency: implications for immune control.." J Immunol. Vol. 174, (January 2005.): 4972-4978.

Flaño, E., Basak, K., Woodland, D.L. and Blackman, M.A.. "Infection of dendritic cells by a γ-herpesvirus induces functional modulation.." J Immunol. Vol. 175, (January 2005.): 3225-3234.

Martinez-Sobrido, L., Gitiban, N., Fernandez-Sesma, A., Cros, J., Mertz, S.E., Jewell, N.A., Hammond, S., Flaño, E., Durbin, R.K., Garcia-Sastre, A., Durbin, J.E.. "Protection against Respiratory Syncytial Virus by a Recombinant Newcastle Disease Virus Vector.." J Virol. Vol. 80, (January 2006.): 1130-1139.

Weslow-Schmidt JL, Jewell NA, Mertz SE, Simas JP, Durbin JE, Flaño E. "Type I interferon inhibition and dendritic cell activation during gammaherpesvirus respiratory infection." J Virol. Vol. 81, no. 18. (January 2007.): 9778-9789.

Jewell NA, Vaghefi N, Mertz SE, Akter P, Peebles RS Jr, Bakaletz LO, Durbin RK, Flaño E, Durbin JE. "Differential type I interferon induction by respiratory syncytial virus and influenza a virus in vivo." J Virol. Vol. 81, no. 18. (January 2007.): 9790-9800.

Cush SS, Anderson KM, Ravneberg DH, Weslow-Schmidt JL, Flaño E. "Memory generation and maintenance of CD8+ T cell function during viral persistence." J Immunol. Vol. 179, no. 1. (January 2007.): 141-153.

Cush,Stephanie,S; Flano,Emilio. "Protective Antigen-Independent CD8 T Cell Memory Is Maintained during gamma-Herpesvirus Persistence." JOURNAL OF IMMUNOLOGY. Vol. 182, no. 7. (April 2009.): 3995-4004.

Stuller, K.A. and Flaño, E. "CD4 T cell-mediated killing in vivo during persistent gammaherpesvirus 68 infection." J Virol. Vol. 83, (January 2009.): 4700-4703.

Hwang, S., Kim, K.S., Flano, E., Wu, T., Tong, L.M., Park, A.N., Song, M.J., Sanchez, D.J., O’Connell, R.M., Cheng, G. and Sun, R.. "Conserved herpesviral kinase promotes viral persistence by inhibiting the IRF-3-mediated type I interferon response." Cell Host & Microbe. Vol. 5, (January 2009.): 166-178.

Stuller,Kathleen,A; Cush,Stephanie,S; Flano,Emilio. "Persistent gamma-Herpesvirus Infection Induces a CD4 T Cell Response Containing Functionally Distinct Effector Populations." JOURNAL OF IMMUNOLOGY. Vol. 184, no. 7. (April 2010.): 3850-3856.

Stuller,K,A; CUSH,S,S; Flano,E. "Persistent gamma-herpesvirus infection induces a CD4 T cell response containing functionally distinct effector populations (vol 184, pg 3850, 2010)." JOURNAL OF IMMUNOLOGY. Vol. 184, no. 12. (June 2010.): 7315-7315.

Weslow-Schmidt,Janet; Ye,Fang; Cush,Stephanie,S; Stuller,Kathleen,A; Blackman,Marcia,A; Flano,Emilio. "Dendritic Cells Loaded with Tumor B Cells Elicit Broad Immunity against Murine Gammaherpesvirus 68 but Fail To Prevent Long-Term Latency." JOURNAL OF VIROLOGY. Vol. 84, no. 17. (September 2010.): 8975-8979.

Jewell NA, Cline T, Mertz SE, Smirnov SV, Flano E, Schindler C, Grieves JL, Durbin RK, Kotenko SV, Durbin JE.. "Lambda interferon is the predominant interferon induced by influenza A virus infection in vivo.." J Virol. Vol. 84, no. 21. (January 2010.): 11515-11522.

Jewell,Nancy,A; Cline,Troy; Mertz,Sara,E; Smirnov,Sergey,V; Flano,Emilio; Schindler,Christian; Grieves,Jessica,L; Durbin,Russell,K; Kotenko,Sergei,V; Durbin,Joan,E. "Lambda Interferon Is the Predominant Interferon Induced by Influenza A Virus Infection In Vivo (vol 84, pg 11515, 2010)." JOURNAL OF VIROLOGY. Vol. 85, no. 1. (January 2011.): 650-650.

Cush,Stephanie,S; Flano,Emilio. "KLRG1(+)NKG2A(+) CD8 T Cells Mediate Protection and Participate in Memory Responses during gamma-Herpesvirus Infection." JOURNAL OF IMMUNOLOGY. Vol. 186, no. 7. (April 2011.): 4051-4058.

Freeman,Michael,L; Burkum,Claire,E; Lanzer,Kathleen,G; Jensen,Meghan,K; Ahmed,Mushtaq; Yager,Eric,J; Flano,Emilio; Winslow,Gary,M; Woodland,David,L; Blackman,Marcia,A. "Cutting Edge: Activation of Virus-Specific CD4 T Cells throughout gamma-Herpesvirus Latency." JOURNAL OF IMMUNOLOGY. Vol. 187, no. 12. (December 2011.): 6180-6184.

Aeffner F, Bratasz A, Flaño E, Powell KA, Davis IC.. "Post-Infection A77-1726 Treatment Improves Cardiopulmonary Function in H1N1 Influenza-Infected Mice.." Am J Respir Cell Mol Biol.. Vol. 47, no. 4. (June 2012.): 543-51.

Fang, Y. Turner, J., Flaño E. 2012. "Contribution of Pulmonary KLRG1 high and KLRG1 low CD8 T Cells to Effector and Memory Responses during Infuenza Virus Infection.." Journal of Immunology. Vol. 189, no. 11. (October 2012.): 5206-11.

McNally, B., Willette, M., Partida-Sanchez, S., Flaño, E.. "Intranasal administration of dsRNA induces interferon- receptor-dependent accumulation of antigen experienced T cells in the airways.." Plos One. Vol. 7, no. 12. (December 2012.): e51351-.

Ioannidis I, McNally B, Willette M, Peeples ME, Chaussabel D, Durbin JE, Ramilo O, Mejias A, Flano E. "Plasticity and virus-specificity of the airway epithelial cell immune response during respiratory viral infection." J Virol. Vol. 86, no. 10. (March 2012.): 5422-36.

Betebibel, S.-E., Lopez, S., Obermoser, G., Schmitt, N., Mueller, C., Harrod, C., Flano, E., Mejias, A., Albrecht, R.A., Blankenship, D., Xu, H., Pascual, V, Bancherau, J., Garcia-Sastre, A., Palucka, K., Ramilo, O., Ueno, H.. "Inductin of ICOS+CXCR3+CXCR5+ TH cells correlates with antibody responses to influenza vaccination.." Sci Transl Med. Vol. 5, no. 176. (March 2013.): 176ra32-.

McNally B, Ye F, Willette M, Flanoe E. "Local blockade of epithelial PDL-1 in the airways enhances T cell function and viral clearance during influenza virus infection." J Virol. Vol. 87, no. 23. (December 2013.): 12916-24.

Ioannidis I, Ye F, McNally B, Willette M, Flaño E.. "TLR expression and induction of type I and type III interferons in primary airway epithelial cells.." J Virol. Vol. 87, no. 6. (January 2013.): 3261-3270.

Cyktor, J, Caruthers, B, Stromberg, P, Flano, E, Pircher, H, Turner, E.. "KLRG1 deficiency significantly enhances survival after Mycobacterium tuberculosis infection.." Infec Immun. Vol. 81, no. 4. (January 2013.): 1090-9.

Cao RG, Suarez NM, Obermoser G, Lopez SM, Flano E, Mertz SE, Albrecht RA, Garcia-Sastre A, Mejias A, Xu H, Qin H, Blankenship D, Palucka K, Pascual V, Ramilo O. "Differences in antibody responses between TIV and LAIV influenza vaccines correlate with the kinetics and magnitude of interferon signaling in children." J Infect Dis. Vol. Epub ahead of print Feb 4, (February 2014.): na-.

 

Reference Works

January 2009 Cush SS, Flano E."Modulation of memory CD8 T cell function through the inhibitory receptors NKG2A and KLRG1" .
January 2009 Cush SS, Flano E."Modulation of memory CD8 T cell function through the inhibitory receptors NKG2A and KLRG1" .
January 2009 Cush SS, Flano E."Modulation of memory CD8 T cell function through the inhibitory receptors NKG2A and KLRG1" .
January 2010 Cush, S.S., Flano, E.."KLRG1+NKG2A+ CD8 T cell response during gammaherpesvirus infection" .
January 2010 Cush, S.S., Flano, E.."KLRG1+NKG2A+ CD8 T cell response during gammaherpesvirus infection" .
January 2010 Cush, S.S., Flano, E.."KLRG1+NKG2A+ CD8 T cell response during gammaherpesvirus infection" .
January 2010 Cush, S.S., Flano, E.."KLRG1+NKG2A+ CD8 T cell response during gammaherpesvirus infection" .
January 2011 Ye, F, Flano, E.."Proliferation of airway CD8 T cells during primary and secondary influenza virus infection" .
January 2011 McNally, B., Ye, F., Willette, M., Durbin, J.E., Flano, E.."Type I interferon signaling control lung T lymphocyte transmigration into the airways" .
January 2011 McNally, B., Ye, F., Willette, M., Durbin, J.E., Flano, E.."Type I interferon signaling controls T lymphocyte transmigration into the airways" Vancouver.
October 2011 Lopez S, Flano E, Obermoser G, Ranganathan R, Mertz S, Farley S, Davies P, Garcia-Sastre A, Albretcht R, Mejias A, Ramilo O."na" Boston.
May 2011 Lopez S, Flano E, Mejias A, Ranganathan R, Mertz S, Ramilo O."na" Denver.
January 2011 Willette, M. Ioannidis, I., Flano, E.."Pathogen Recognition by Airway Epithelial Cells" .
January 2011 Ioannidis, I., McNally, B., Willette, M., Mejias, A., Flano, E.."Plasticity of the airway epithelial cell response during respiratory virus infection" .
May 2012 Lopez S, Suarez MC, Mertz S, Mella C, Arthur G, Flano E, Ramilo O and Mejias A."na" Boston.
October 2012 Lopez S, Flano E, Suarez-Arrabal MC, Mertz S, Mella C, Dimo B, Arthur G, Chaussabel D, Ramilo O, Mejias A.."na" San Diego.
September 2012 Ortiz-Marty R J, Ye F, Mejias A, Ramilo O and Flano E."na" Santa Fe.
October 2012 Cao R, Lopez S, Flano E, Obermoser G, Mertz S, Farley S, Davies P, Garcia-Sastre A, Albretcht R, Mejias A, Ramilo O."na" San Diego.

Professional Activities

present NIH Scientific Review Group ZRG1 BBBP-E (53). NIH. Bethesda, MD.
present NIH Scientific Review Group ZOD1 CM-5 (01)1. NIH. Bethesda, MD.
present NIH Scientific Review Group ZAI1 QV-I M1 1. NIH. Bethesda, MD.
present NIH Immunity and Host Disease Study Section. NIH. Bethesda, MD.
2011 - present NIH Scientific Review Group, ZAi1 QV-I (S1) 1 Special Emphasis Panel. National Institute of Health.
2011 - present NIH Scientific Review Group, ZAi1 QV-I (M3) 1 Special Emphasis Panel. National Institute of Health.
2011 - present External Grant Reviewer. The University of Nebraska Lincoln.
2012 - present NIH Scientific Review Group ZAI1 QV-I M3 1. NIH. Bethesda, MD.