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Dmitri Kudryashov, PhD


484 W 12th Ave
Columbus, OH 43210

Phone: (614) 292-4848

Email: kudryashov.1@osu.edu

 

Current OSU Appointment

Associate Professor, Chemistry and Biochemistry

 

Noteworthy Graduate Narrative

Graduate student researchers in the Kudryashov Lab presented their research at local and international conferences, co-authored publications in scientific journals, won scholarships and awards for presentations emanating from their research. A brief summary of students' accomplishments is specified below.

Dissertation advisor, David Heisler (OSBP)
- 2016 PHPID Fellowship winner
- Poster presentation at the 2016 IGP Symposium
- Oral presentation at the 2016 OBAMS meeting
- Poster presentation and a Moderator at the 2016 CMIB symposium
- 1st place in Oral Presentations at the Hayes Graduate Research Forum 2016
- “Best Poster Award” at the OSU PHPID 2015 annual meeting
- student moderator for the “Parallel Student Talks Session” at the 2015 OSU IGP Symposium
- 2nd place Poster Award at the Dow Graduate Student Poster Competition 2015
- plenary speaker at the 2014 OSU Molecular Life Sciences Interdisciplinary Graduate Programs Symposium
- poster presentation at 2013, 2014, and 2015 ASCB/IFCB International Meetings
- president of the Ohio State Biochemistry Program
- co-author of one published paper (Kudryashova et al., Molecular Microbiology 2014)
- first author manuscript (Heisler et al., Science 2015)
- co-authored a book chapter (Current Topics in Microbiology and Immunology - Springer; in press)
- two manuscripts in preparation

Dissertation advisor, Christopher Schwebach (MCDB)
- Poster presentation at the 2016 IGP Symposium
- Poster presentation at the 2016 CMIB symposium
- Poster presentation at the 2016 Edward F. Hayes Graduate Research Forum
- Poster presentation at 2014 and 2015 ASCB/IFCB International Meetings
- two manuscripts in preparation

Advising Narrative

Undergraduate student researchers in the Kudryashov Lab have won awards for research activities and poster presentations (including Denman), as well as grants and fellowships (including Pelotonia and ASC Honors) for carrying out research projects; they co-authored research papers. A brief summary of the students' achievements is highlighted below.

Research advisor, Richa Agrawal (current student, Biochemistry major)
- 2016 Spring ASC Honors Undergraduate Research Scholarship
- Mayers Summer Research Scholarship (summer 2016)
- Undergraduate Research Scholarship (summer 2015)
- poster at Denman forum, 2016

Senior thesis advisor, Mubarik Mohamed (current student, Microbiology major)
- Undergraduate Pelotonia winner (2016)
- a stipendiat of REU program sponsored by The National Science Foundation (Summer 2014)
- posters at local forums (8th Annual Fall 2014 Undergraduate Research Week and Student Poster Forum and Denman 2015 and 2016)

Research advisor, Blake Williams (current student, Biochemistry major)
- 3rd place for a poster presentation at The ACS (American Chemical Society) Columbus Section and OSU Chemistry & Biochemistry Club Research Forum
- The Bertram Thomas Memorial Scholarship by the CBC department for the 2015-2016 school year
- poster at Denman forum, 2016
- manuscript in preparation

Senior thesis advisor, Kyle Shafer (graduated in Spring 2016, Biochemistry major)
- 1st place winner at The 2016 Denman Undergraduate Research Forum
- The Bertram Thomas Memorial Scholarship by the CBC department for the 2015-2016 school year
- graduated with Honors Research Distinction
- manuscript in preparation

Research advisor, Samantha Pawela (graduated in Spring 2015, Neuroscience major)
- ASC Honors Undergraduate Research Scholarship (Autumn 2014)
- poster at Denman forum, 2015
- currently a medical student (Kansas City University School of Medicine and Biosciences)

Senior thesis advisor, Dmitry Grinevich (graduated in Spring of 2014, Biochemistry major)
- ASC Undergraduate Research Scholarship (Autumn 2013)
- outstanding poster Award at NMS research forum 2014
- the 2014 Denman Undergraduate Research Forum Winner (2nd place – Biological Sciences)
- co-author of a manuscript (Heisler et al., Science 2015)
- graduated with Honors Research Distinction
- currently a graduate student at North Carolina State University; graduate NSF scholarship recipient

Research advisor, Andrew Zywiec (graduated in Spring of 2014, Biology major)
- the 2014 Denman Undergraduate Research Forum Winner (1st place – Biological Sciences)
- co-author of a manuscript (Kudryashova et al., Molecular Microbiology 2014)
- currently a medical student at St. George's University Medical School

Senior thesis advisor, Barry Golestany (graduated in Spring of 2013, Biochemistry major)
- presented a poster at Denman forum, 2013
- graduated with Honors Research Distinction
- currently a medical student at Rosalind Franklin Medical School

High school student research advisor, Cassidy Wang
- presented a poster at the departmental Early Experience in Science (Biochem 2998H forum (2014)
- manuscript in preparation
- currently a student at Georgia Tech, Bioengineering major; REU NSF program scholar (Summer 2016)

Published Works

Publications since arrival to OSU (2011-present):
14 (including 1 book chapter in-press - item c1, 11 articles items e1-11, 1 invited editorial item f1, and 1 review item g1).
Two articles published in highly ranked journals: SCIENCE (IF: 34.7 - item e2) and IMMUNITY (IF: 21.6 - item e6).
Manuscripts currently submitted: 2.
Manuscripts in preparation: 3 (including one invited review).

Total peer/editor reviewed journal articles and reviews: 35

Research Funding

Research support has been provided by several agencies at the national and local levels to Dr. Kudryashov and members of his group.

Funding to Kudryashov group members:
Graduate fellowships:

- PHPID Graduate scholarship.
Undergraduate fellowships:
- Mayers Summer Research Scholarship
- Research Scholar Awards
- Arts and Sciences Honors Research Scholarships
- Pelotonia

Academic Advising

2012 Zihua Zhu,, The Ohio State University. Graduated 0.
2012 David Heisler,, The Ohio State University. Graduated 0. "Novel mechanisms of toxicity amplification: poisoning actin binding protein-controlled actin polymerization"
2013 Christopher Schwebach,, The Ohio State University. Graduated 0. "Elucidating mechanisms of cancer metastasis mediated by the dysregulation of plastin-2"
2013 Walter Zahurancik,, The Ohio State University. Graduated 0.
2013 Jenna Yehl,, University of Delaware. Graduated 0.
2014 Mubarik Mohamed, The Ohio State University. Graduated 0. "Regulation of F-actin bundling by human plastins"
2014 Vibhuti Wadhwa,, The Ohio State University. Graduated 0.
2014 Doha Wang,, The Ohio State University. Graduated 0.
2014 Anthony Stephenson,, The Ohio State University. Graduated 0.
2014 She Shu,, The Ohio State University. Graduated 0.
2014 Yancheng Hu,, The Ohio State University. Graduated 0.
2015 Chase McVey,, The Ohio State University. Graduated 0.
2015 Jin Wen,, The Ohio State University. Graduated 0.
2015 Vedud Purde,, The Ohio State University. Graduated 0. "Novel approach for selective elimination of cancer cells by dual targeting with bacterial split-toxins"
2015 Blake Williams, The Ohio State University. Graduated 0.
2015 Yajun Liu, The Ohio State University. Graduated 0. "Roles of a novel protein Rng14 in cytokinesis and cell polarity"
2015 Richa Agrawal, The Ohio State University. Graduated 0.
2016 Cristian Arias, Bloomfield College. Graduated 0.
2016 Jonathan Wright, The Ohio State University. Graduated 0.
2016 Alex Prokup, The Ohio State University. Graduated 0.
2011 Emily Wong,, The Ohio State University. Graduated 2011.
2011 Michael Yingling, The Ohio State University. Graduated 2011.
2011 - 2012 Andrei Jipa, The Ohio State University. Graduated 2014.
2011 - 2012 Tess Keppler, The Ohio State University. Graduated 2013.
2012 Sneha Gupta,, The Ohio State University. Graduated 2012. "Targeting Protein Metabolism in B-cell Malignancies"
2012 - 2013 Brian Meyerson, The Ohio State University. Graduated 2014.
2013 Dominic Julian, The Ohio State University. Graduated 0.
2013 , The Ohio State University.
2012 - 2014 Cassidy Wang, Dublin Jerome High School. Graduated 0. High school student
2012 - 2014 Barry Golestany, The Ohio State University. Graduated 2013. "Screening for Actin-Binding-Proteins Selectively Interacting With Undecorated and Cofilin-Decorated Actin"
2013 - 2014 Andrew Zywiec, The Ohio State University. Graduated 2014.
2013 - 2014 Dmitry Grinevich, The Ohio State University. Graduated 2014. "The effects of ACD crosslinked actin-oligomers on mDia1 mediated actin polymerization"
2014 Lauren Strange, The Ohio State University. Graduated 2014.
2014 , The Ohio State University.
2014 Nathan Dissinger,, The Ohio State University. Graduated 2014. “Role of HTLV-1 HBZ and HTLV-2 APH-2 in Disease Outcome�
2013 - 2015 Samantha Pawela, The Ohio State University. Graduated 2015.
2014 - 2015 Royston Quintyn,, The Ohio State University. Graduated 2015. “Applying Tandem Mass Spectrometry coupled with Ion Mobility to probe the Structure of Non-Covalent Protein Complexes and their Interactions with Ligands, Peptides and other Proteins�
2014 - 2015 Dylan Bederman, The Ohio State University. Graduated 0.
2014 - 2015 Matt Saab, The Ohio State University. Graduated 2015. "Controllable Inactivation of a Toxin-based Tool to Target Actin in vivo"
2015 Vivian Jung,, The Ohio State University. Graduated 0.
2013 - 2016 Kyle Shafer, The Ohio State University. Graduated 0. "ACD-crosslinked actin oligomers act as dose-dependent inhibitors of Ena/VASP mediated actin polymerization"
2014 - 2016 Sean Gill, The Ohio State University. Graduated 0.
2015 - 2016 Chase Smith, The Ohio State University. Graduated 0.
2016 Mangla Shubham,, The Ohio State University. Graduated 2016.
 

Chapters in Books

Kudryashova E, Heisler DB, Kudryashov DS. "Pathogenic mechanisms of actin cross-linking toxins – peeling away the layers." In Current Topics in Microbiology and Immunology - Springer. -. January 0.

 

Conferences

Muhlrad,A; Kudryashov,D; Andrey,B,A; Steven,A,C; Reisler,E. "Cofilin exposes subdomain 2 on F-actin to proteolysis." In BIOPHYSICAL JOURNAL. (January 2004). 68A-68A.

Kudryashov,D,S; Phillips,M; Reisler,E. "Formation and destabilization of actin filaments with tetramethylrhodamine-modified actin." In BIOPHYSICAL JOURNAL. (August 2004). 1136-1145.

Kudryashov,D,S; Pavlov,D,A; Bates,R,B; Reisler,E. "Comparison of phalloidin and dolastatin 11 effects on F-actin stabilization and cofilin binding." In BIOPHYSICAL JOURNAL. (January 2004). 70A-70A.

Orlova,A; Shvetsov,A; Galkin,V,E; Kudryashov,D; Rubenstein,P,A; Reisler,E; Egelman,E,H. "New insights into actin filaments dynamics." In BIOPHYSICAL JOURNAL. (January 2005). 496A-496A.

Kudryashov,D,S; Galkin,V,E; Orlova,A; Egelman,E,H; Reisler,E. "Cofilin promotes filament assembly of polymerization-impaired actin." In BIOPHYSICAL JOURNAL. (January 2005). 496A-496A.

Dmitri S. Kudryashov, Zeynep A. Oztug-Durer, A Jimmy Ytterberg, Michael R. Sawaya, Inna Pashkov, Tod O. Yeates, Rachel Ogorzalek Loo, Joseph Loo, Karla J. Satchell, Emil Reisler. "Covalent cross-linking of actin by the ACD domain of Vibrio Cholerae MARTX toxin." In BIOPHYSICAL JOURNAL. (February 2008). Supplement, p44, 143-Plat.

Galkin,V,E; Orlova,A; Kudryashov,D; Soloduhin,A; Reisler,E; Schroeder,G; Egelman,E,H. "Remodeling of Actin Filaments by Cofilin and Phalloidin." In MOLECULAR BIOLOGY OF THE CELL. (January 2011). A-.

Butterworth,Andrew,H; Yan,Si; Kudryashov,Dmitri; Reisler,Emil; Polenova,Tatyana. "Fast Magic Angle Sample Spinning NMR Yields a View of the F-actin - Cofilin Complex with Atomic Resolution." In BIOPHYSICAL JOURNAL. (February 2011). 300-300.

Galkin,Vitold,E; Orlova,Albina; Kudryashov,Dmitri; Soloduhin,Alexandr; Reisler,Emil; Schroeder,Gunnar,F; Egelman,Edward,H. "Remodeling of Actin Filaments by Cofilin." In BIOPHYSICAL JOURNAL. (January 2012). 238A-238A.

Audette,Nicholas,Jack; Yan,Si; Kudryashov,Dmitri; Polenova,Tatyana. "NMR Analysis of Actin Binding Protein Cofilin." In FASEB JOURNAL. (April 2012). A-.

Vilitkevich,E,L; Khapchaev,A,Y; Kudryashov,D,S; NIKASHIN,A,V; Schavocky,J,P; Lukas,T,J; Watterson,D,M; Shirinsky,V,P. "Phosphorylation regulates interaction of 210-kDa myosin light chain kinase N-terminal domain with actin cytoskeleton." In BIOCHEMISTRY-MOSCOW. (October 2015). 1288-1297.

Serebryannyy,Leo; Parilla,Megan; Annibale,Paolo; Cruz,Christina; Kudryashov,Dmitri; Gratton,Enrico; Gottardi,Cara,J; de Lanerolle,Primal. "Nuclear Actin Dynamics Regulate Nuclear Organization and Transcription." In BIOPHYSICAL JOURNAL. (January 2015). 536A-536A.

 

Degrees

1995 M.D., Russian State Medical University

2000 Ph.D., Russian Academy of Medical Science

 

Editorial Activities

2011 The Research Grants Council of Hong Kong
2013 - present National Innovative Research Grant (IRG), American Heart Association
2014 - present Microbial Pathogenesis
2014 - present Molecular Microbiology
2014 - present Toxins
2015 - present Applied Microbiology and Biotechnology
2015 - present Pathogens and Disease
2015 - present Biophysical Journal
2015 - present Biochimica et Biophysica Acta
2015 - present Nature Communications
2015 The Research Grants Council of Hong Kong
2016 - present Molecular Biology - Springer
 

Honors

2004 - 2006 AHA Postdoctoral Award. American Heart Association.
2005 - present Departmental Herbert Newby McCoy Award for the Greatest Contribution of the Year to the Science of Chemistry. Department of Chemistry and Biochemistry.
2005 - present Boyer-Peter Award for outstanding postdoctoral studies in Biochemistry. Department of Chemistry and Biochemistry.
2005 - present Postdoctoral Recognition Award. Department of Chemistry and Biochemistry.
2012 - 2014 Innovative Research Grant. American Heart Association.
2013 - 2014 Innovative Concept Award. U.S. Department of Defense.
2015 - present Nomination for a Distinguished Undergraduate Research Mentor Award. Undergraduate Research Office.
2015 - present ASCB-Gibco Emerging Leader Prize Finalist. GIBCO.
2015 - present Young Investigator Award. BBA Biomembranes.
2016 - present PATH Award Finalist. Burroughs Wellcome Fund.
2016 - present Nomination for a Distinguished Undergraduate Research Mentor Award. Undergraduate Research Office.
2016 - present Two research articles were listed within the Top 10 Most Impactful Papers published within 2014-2016 by the faculties of the Center for Microbial Interface Biology, OSU (#3 - Science PMID: 26228148; #4 - Immunity PMCID: PMC4269836). CMIB.
 

Journal Articles

Kudryashov DS, Chibalina MV, Birukov KG, Lukas TJ, Sellers JR, Van Eldik LJ, Watterson DM; Shirinsky VP. "Unique sequence of a high molecular weight myosin light chain kinase is involved in interaction with actin cytoskeleton." FEBS LETTERS. Vol. 463, no. 1-2. (December 1999.): 67-71.

Bushueva TL, Teplova MV, Bushuev VN, Kudriashov DS, Vorotnikov AV, Shirinsky VP. "Stability of the structure of KRP (kinase-related protein).." MOLECULAR BIOLOGY (RUS). Vol. 33, no. 2. (March 1999.): 227-236.

Chibalina MV, Kudriashov DS, Shekhonin BV, Shirinsky VP. "Functional properties and intracellular localization of high molecular weight isoform of myosin light chain kinase.." TSITOLOGIA (RUS). Vol. 42, no. 3. (January 2000.): 248-255.

Vorotnikov AV, Krymsky MA, Chibalina MV, Kudriashov DS, Shirinsky VP. "Differences in contraction and regulatory protein phosphorylation of phasic and tonic smooth muscles.." TSITOLOGIA (RUS). Vol. 42, no. 4. (February 2000.): 378-391.

Krymsky,M,A; Kudryashov,D,S; Shirinsky,V,P; Lukas,T,J; Watterson,D,M; Vorotnikov,A,V. "Phosphorylation of kinase-related protein (telokin) in tonic and phasic smooth muscles." JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY. Vol. 22, no. 5. (January 2001.): 425-437.

Kudryashov,D,S; Vorotnikov,A,V; Dudnakova,T,V; Stepanova,O,V; Lukas,T,J; Sellers,J,R; Watterson,D,M; Shirinsky,V,P. "Smooth muscle myosin filament assembly under control of a kinase-related protein (KRP) and caldesmon." JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY. Vol. 23, no. 4. (January 2002.): 341-351.

Kudryashov D.S., Reisler E.. "Solution properties of tetramethylrhodamine-modified G-actin." BIOPHYSICAL JOURNAL. Vol. 85, no. 4. (October 2003.): 2466-2475.

Muhlrad,A; Kudryashov,D; Peyser,Y,M; Bobkov,A,A; Almo,S,C; Reisler,E. "Cofilin induced conformational changes in F-actin expose subdomain 2 to proteolysis." JOURNAL OF MOLECULAR BIOLOGY. Vol. 342, no. 5. (October 2004.): 1559-1567.

Orlova,A; Shvetsov,A; Galkin,V,E; Kudryashov,D,S; Rubenstein,P,A; Egelman,E,H; Reisler,E. "Actin-destabilizing factors disrupt filaments by means of a time reversal of polymerization." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. Vol. 101, no. 51. (December 2004.): 17664-17668.

Vilitkevich EL, Kudryashov DS, Stepanova OV, Shirinsky VP. "A new actinbinding area of the myosin light chains’ high-molecular kinase.." ROSS. FIZIOL.ZH. IM. IM SECHENOVA (RUS). Vol. 90, no. 5. (May 2004.): 577-585.

Kudryashov,D,S; Phillips,M; Reisler,E. "Formation and destabilization of actin filaments with tetramethylrhodamine-modified actin." BIOPHYSICAL JOURNAL. Vol. 87, no. 2. (August 2004.): 1136-1145.

Kudryashov D.S., Stepanova O.V., Vilitkevich E.L., Nikonenko T.A., Nadezhdina E.S., Shanina N.A., Lukas T.J., Van Eldik L.J., Watterson D.M., Shirinsky V.P.. "Myosin light chain kinase (210 kDa) is a potential cytoskeleton integrator through its unique N-terminal domain.." EXPERIMENTAL CELL RESEARCH. Vol. 298, no. 2. (August 2004.): 407-417.

Kudryashova,E; Kudryashov,D; Kramerova,I; Spencer,M,J. "Trim32 is a ubiquitin ligase mutated in limb girdle muscular dystrophy type 2H that binds to skeletal muscle myosin and ubiquitinates actin." JOURNAL OF MOLECULAR BIOLOGY. Vol. 354, no. 2. (November 2005.): 413-424.

Kudryashov,D,S; Sawaya,M,R; Adisetiyo,H; Norcross,T; Hegyi,G; Reisler,E; Yeates,T,O. "The crystal structure of a cross-linked actin dimer suggests a detailed molecular interface in F-actin." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. Vol. 102, no. 37. (September 2005.): 13105-13110.

Cordero,Christina,L; Kudryashov,Dmitry,S; Reisler,Emil; Satchell,Karla,JFullner. "The actin cross-linking domain of the Vibrio cholerae RTX toxin directly catalyzes the covalent cross-linking of actin." JOURNAL OF BIOLOGICAL CHEMISTRY. Vol. 281, no. 43. (October 2006.): 32366-32374.

Kudryashov,D,S; Galkin,V,E; Orlova,A; PHAN,M; Egelman,E,H; Reisler,E. "Cofilin cross-bridges adjacent actin protomers and replaces part of the longitudinal F-actin interface." JOURNAL OF MOLECULAR BIOLOGY. Vol. 358, no. 3. (May 2006.): 785-797.

Sawaya,Michael,R; Kudryashov,D,S; Pashkov,Inna; Adisetiyo,Helty; Reisler,Emil; Yeates,Todd,O. "Multiple crystal structures of actin dimers and their implications for interactions in the actin filament." ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY. Vol. 64, (April 2008.): 454-465.

Kudryashov,Dmitri,S; Cordero,Christina,L; Reisler,Emil; Satchell,Karla,JFullner. "Characterization of the enzymatic activity of the actin cross-linking domain from the Vibrio cholerae MARTX(Vc) toxin." JOURNAL OF BIOLOGICAL CHEMISTRY. Vol. 283, no. 1. (January 2008.): 445-452.

Kudryashov,Dmitri,S; Durer,Zeynep,AOztug; Ytterberg,A,Jimmy; Sawaya,Michael,R; Pashkov,Inna; Prochazkova,Katerina; Yeates,Todd,O; Loo,Rachel,ROgorzalek; Loo,Joseph,A; Satchell,Karla,JFullner; Reisler,Emil. "Connecting actin monomers by iso-peptide bond is a toxicity mechanism of the Vibrio cholerale MARTX toxin." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. Vol. 105, no. 47. (November 2008.): 18537-18542.

Durer,Zeynep,AOztug; Diraviyam,Karthikeyan; Sept,David; Kudryashov,Dmitri,S; Reisler,Emil. "F-Actin Structure Destabilization and DNase I Binding Loop Fluctuations Mutational Cross-Linking and Electron Microscopy Analysis of Loop States and Effects on F-Actin." JOURNAL OF MOLECULAR BIOLOGY. Vol. 395, no. 3. (January 2010.): 544-557.

Grintsevich,Elena,E; Galkin,Vitold,E; Orlova,Albina; Ytterberg,A,Jimmy; Mikati,Mouna,M; Kudryashov,Dmitri,S; Loo,Joseph,A; Egelman,Edward,H; Reisler,Emil. "Mapping of Drebrin Binding Site on F-Actin." JOURNAL OF MOLECULAR BIOLOGY. Vol. 398, no. 4. (May 2010.): 542-554.

Kudryashov,Dmitri,S; Grintsevich,Elena,E; Rubenstein,Peter,A; Reisler,Emil. "A Nucleotide State-sensing Region on Actin." JOURNAL OF BIOLOGICAL CHEMISTRY. Vol. 285, no. 33. (August 2010.): 25591-25601.

Galkin,Vitold,E; Orlova,Albina; Kudryashov,Dmitri,S; Solodukhin,Alexander; Reisler,Emil; Schroeder,Gunnar,F; Egelman,Edward,H. "Remodeling of actin filaments by ADF/cofilin proteins." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. Vol. 108, no. 51. (December 2011.): 20568-20572.

Durer,Zeynep,A; Kudryashov,Dmitri,S; Sawaya,Michael,R; Altenbach,Christian; Hubbell,Wayne; Reisler,Emil. "Structural States and Dynamics of the D-Loop in Actin." BIOPHYSICAL JOURNAL. Vol. 103, no. 5. (September 2012.): 930-939.

Kudryashova,Elena; Kalda,Caitlin; Kudryashov,Dmitri,S. "Glutamyl Phosphate Is an Activated Intermediate in Actin Crosslinking by Actin Crosslinking Domain (ACD) Toxin." PLOS ONE. Vol. 7, no. 9. (September 2012.): e45721-.

Kudryashov,Dmitri,S; Reisler,Emil. "ATP and ADP actin states." BIOPOLYMERS. Vol. 99, no. 4. (April 2013.): 245-256.

Lyon,Alison,N; Pineda,Ricardo,H; Le Thi Hao,L,T; Kudryashova,Elena; Kudryashov,Dmitri,S; Beattie,Christine,E. "Calcium binding is essential for plastin 3 function in Smn-deficient motoneurons." HUMAN MOLECULAR GENETICS. Vol. 23, no. 8. (April 2014.): 1990-2004.

Kudryashova E, Quintyn RS, Lu W, Seveau S, Wysocki V, Kudryashov DS. "(2014) Human defensins facilitate local unfolding of thermodynamically unstable regions of bacterial protein toxins." Immunity. Vol. 41, no. 5. (November 2014.): 709-21.

Ge P., Oztug Durer Z.A., Kudryashov D.S., Zhou Z.H., Reisler E.. "Cryo-EM reveals different coronin binding modes for ADP- and ADP-BeFx actin filaments.." NATURE STRUCTURAL & MOLECULAR BIOLOGY. Vol. 21, no. 12. (December 2014.): 1075-1081.

Ge,Peng; Durer,Zeynep,AOztug; Kudryashov,Dmitri; Zhou,Z,Hong; Reisler,Emil. "Cryo-EM reveals different coronin binding modes for ADP- and ADP-BeFx actin filaments." NATURE STRUCTURAL & MOLECULAR BIOLOGY. Vol. 21, no. 12. (December 2014.): 1075-1081.

Kudryashova,Elena; Quintyn,Royston; Seveau,Stephanie; Lu,Wuyuan; Wysocki,Vicki,H; Kudryashov,Dmitri,S. "Human Defensins Facilitate Local Unfolding of Thermodynamically Unstable Regions of Bacterial Protein Toxins." IMMUNITY. Vol. 41, no. 5. (November 2014.): 709-721.

Kudryashova,Elena; Heisler,David; Zywiec,Andrew; Kudryashov,Dmitri,S. "Thermodynamic properties of the effector domains of MARTX toxins suggest their unfolding for translocation across the host membrane." MOLECULAR MICROBIOLOGY. Vol. 92, no. 5. (June 2014.): 1056-1071.

Heisler,David,B; Kudryashova,Elena; Grinevich,Dmitry,O; Suarez,Cristian; Winkelman,Jonathan,D; Birukov,Konstantin,G; Kotha,Sainath,R; Parinandi,Narasimham,L; Vavylonis,Dimitrios; Kovar,David,R; Kudryashov,Dmitri,S. "ACTIN-DIRECTED TOXIN. ACD toxin-produced actin oligomers poison formin-controlled actin polymerization.." Science. Vol. 349, no. 6247. (July 2015.): 535-539.

Kudryashova E, Lu W, Kudryashov DS. "Defensins versus pathogens: an unfolding story." ONCOTARGET. Vol. 6, no. 30. (October 2015.): 28533-28534.

Kudryashova,Elena; Seveau,Stephanie; Lu,Wuyuan; Kudryashov,Dmitri,S. "Retrocyclins neutralize bacterial toxins by potentiating their unfolding." BIOCHEMICAL JOURNAL. Vol. 467, (April 2015.): 311-320.

Kudryashova,Elena; Lu,Wuyuan; Kudryashov,Dmitri,S. "Defensins versus pathogens: an unfolding story." ONCOTARGET. Vol. 6, no. 30. (October 2015.): 28533-28534.

Wang, N., Wang, M., Zhu, Y.H., Grosel, T.W., Sun, D., Kudryashov, D.S., Wu, J.Q.. "The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis." MOLECULAR BIOLOGY OF THE CELL. Vol. 26, no. 2. (January 2015.): 238-255.

Kudryashova, E., Seveau, S., Lu, W., Kudryashov, DS.. "Retrocyclins neutralize bacterial toxins by potentiating their unfolding." Biochemical Journal. Vol. 367, no. 2. (April 2015.): 311-320.

Kudryashova,Elena; Seveau,Stephanie; Lu,Wuyuan; Kudryashov,Dmitri,S. "Retrocyclins neutralize bacterial toxins by potentiating their unfolding." BIOCHEMICAL JOURNAL. Vol. 467, (April 2015.): 311-320.

Heisler DB, Kudryashova E, Grinevich DO, Suarez C, Winkelman JD, Birukov KG, Kotha SR, Parinandi NL, Vavylonis D, Kovar DR, Kudryashov DS. "ACD toxin-produced actin oligomers poison formin-controlled actin polymerization." SCIENCE. Vol. 349, no. 6247. (July 2015.): 535-539.

Vilitkevich,E,L; Khapchaev,A,Y; Kudryashov,D,S; NIKASHIN,A,V; Schavocky,J,P; Lukas,T,J; Watterson,D,M; Shirinsky,V,P. "Phosphorylation regulates interaction of 210-kDa myosin light chain kinase N-terminal domain with actin cytoskeleton." BIOCHEMISTRY-MOSCOW. Vol. 80, no. 10. (October 2015.): 1288-1297.

Wang,Ning; Wang,Mo; Zhu,Yi-hua; Grosel,Timothy,W; Sun,Daokun; Kudryashov,Dmitri,S; Wu,Jian-Qiu. "The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis.." Molecular biology of the cell. Vol. 26, no. 2. (January 2015.): 238-255.

Wang, N., Wang, M, Zhu YH, Grosel TW, Sun D, Kudryashov DS, Wu JQ. "The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis." Mol. Biol. Cell. -.

 

Reference Works

December 2012 Kudryashova E, Kalda C, Kudryashov DS."Actin crosslinking by V. cholera ACD toxin: kinetic parameters and the role of highenergy glutamyl-phosphate intermediate" Online ISSN: 1939-4586. : 1027
December 2013 Kudryashova E, Zywiec A, Heisler DB, Kudryashov DS."Structural plasticity of the effector domains of MARTX toxins" Online ISSN: 1939-4586. : 2260
December 2013 Kudryashova E, Lyon AN, Beattie CE, Kudryashov DS."Actin bundling but not binding activity of plastin 3, a protective modifier of spinal muscular atrophy, is Ca2+-sensitive" Online ISSN: 1939-4586. : 1135
December 2013 Heisler DB, Grinevich DO, Kudryashova E, Kovar DR, Kudryashov DS."The inhibitory effect of ACD-crosslinked actin oligomers on mDia1-mediated actin polymerization" Online ISSN: 1939-4586. : 1835
December 2014 Heisler DB, Kudryashova E, Grinevich DO, Kotha S, Winkelman J, Suarez C, Narasimham P, Kovar DR, Kudryashov DS."Potent inhibition of Diaphanous formins by actin oligomers produced by the Actin Crosslinking Domain toxin of V.cholerae" Online ISSN: 1939-4586. : M10
December 2014 Kudryashova E, Quintyn R, Seveau S, Lu W, Wysocki V, Kudryashov DS."Anti-chaperone activity of human α-defensin HNP1 against bacterial toxins" Online ISSN: 1939-4586. : E21
December 2014 Schwebach CL, Mohamed MB, Pawela SN, Kudryashova E, Kudryashov DS."Calcium-regulated actin bundling activity of human plastins" Online ISSN: 1939-4586. : P872
December 2015 C. Schwebach, C. Wang, D. Heisler, B. Golestany, M. Pawlowski, A. Kloczkowski, M. Sotomayor, V. Sirotkin, E. Kudryashova, D.S. Kudryashov."Characterization of Streptomyces griseoflavus cofilin, a prokaryotic member of the eukaryotic ADF/Cofilin family of proteins" Online ISSN: 1939-4586. : P83
December 2015 E. Kudryashova, W. Lu, D.S. Kudryashov."Defensins potentiate unfolding of bacterial toxins and viral proteins" Online ISSN: 1939-4586. : P2388
December 2015 D. Heisler, E. Kudryashova, C. Suarez, J.D. Winkelman, A.J. Harker, D.R. Kovar, D. Vavylonis, D.S. Kudryashov."An uphill battle: how Actin Crosslinking Domain (ACD) toxin turns actin into a poison" Online ISSN: 1939-4586. : P79

Presentations

"Covalent cross-linking of actin by the ACD domain of Vibrio Cholerae MARTX toxin." Presented at Cincinnati Childrens Hospital, Cincinnati, OH, US|USA. (February 2008)

"Covalent cross-linking of actin by the ACD domain of Vibrio Cholerae MARTX toxin." Presented at Biophysical Society Meeting, Motility minisymposium, Long Beach, CA, US|USA. (February 2008)

"Covalent cross-linking of actin by the ACD domain of Vibrio Cholerae MARTX toxin." Presented at Annual Lake Arrowhead Retreat of the Molecular Biology Institute, UCLA, Lake Arrowhead, CA, US|USA. (October 2008)

"Covalent cross-linking of actin by the ACD domain of Vibrio Cholerae MARTX toxin." Presented at Case Western University, Cleveland, OH, US|USA. (December 2008)

"Covalent cross-linking of actin by the ACD domain of Vibrio Cholerae MARTX toxin." Presented at Loyola University of Chicago, Chicago, IL, US|USA. (January 2008)

"Covalent cross-linking of actin by the ACD domain of Vibrio Cholerae MARTX toxin." Presented at Sanford-Burnham Research Institute, La Jolla, CA, US|USA. (April 2009)

"Covalent cross-linking of actin by the ACD domain of Vibrio Cholerae MARTX toxin." Presented at University of Delaware, Department of Chemistry, Newark, DE, US|USA. (December 2010)

"Anti-chaperon activity of human defense peptides against bacterial and viral effector proteins." Presented at Nationwide Children’s Hospital, Battelle Center for Mathematical Medicine, Columbus, OH, US|USA. (June 2012)

"Actin crosslinking by V. cholera ACD toxin: kinetic parameters and the role of highenergy glutamyl-phosphate intermediate." Presented at 2012 ASCB Annual Meeting, San Francisco, CA, US|USA. (December 2012)

"V. cholerae Actin Crosslinking Domain toxin is the a-ligator in actin pools." Presented at University of Illinois at Chicago, Department of Physiology and Biophysics, Chicago, IL, US|USA. (November 2013)

"Structural plasticity of the effector domains of MARTX toxins." Presented at 2013 ASCB Annual Meeting, New Orleans, LA, US|USA. (December 2013)

"Actin bundling but not binding activity of plastin 3, a protective modifier of spinal muscular atrophy, is Calcium-sensitive." Presented at 2013 ASCB Annual Meeting, New Orleans, LA, US|USA. (December 2013)

"The inhibitory effect of ACD-crosslinked actin oligomers on mDia1-mediated actin polymerization." Presented at 2013 ASCB Annual Meeting, New Orleans, LA, US|USA. (December 2013)

"Potent inhibition of Diaphanous formins by actin oligomers produced by the Actin Crosslinking Domain toxin of V.cholerae." Presented at 2014 ASCB/IFCB Meeting, Philadelphia, PA, US|USA. (December 2014)

"Anti-chaperone activity of human α-defensin HNP1 against bacterial toxins." Presented at 2014 ASCB/IFCB Meeting, Philadelphia, PA, US|USA. (December 2014)

"ACD-crosslinked actin oligomers are potent toxins inhibiting formin-mediated actin polymerization." Presented at 2014 Gordon Research Conference (GRC), West Dover, VT, US|USA. (July 2014)

"Calcium-regulated actin bundling activity of human plastins." Presented at 2014 ASCB/IFCB Meeting, Philadelphia, PA, US|USA. (December 2014)

"Actin bundling but not binding activity of plastin 3, a protective modifier of spinal muscular atrophy, is Ca2+-sensitive." Presented at 2014 Gordon Research Conference (GRC), West Dover, VT, US|USA. (July 2014)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at Department of Biomedical Engineering, Columbus, OH, US|USA. (April 2015)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at College of Pharmacy, Columbus, OH, US|USA. (October 2015)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at Edinboro University, Edinboro, PA, US|USA. (April 2015)

"Defensins potentiate unfolding of bacterial toxins and viral proteins." Presented at 2015 ASCB/IFCB Meeting, San Diego, CA, US|USA. (December 2015)

"An uphill battle: how Actin Crosslinking Domain (ACD) toxin turns actin into a poison." Presented at 2015 ASCB/IFCB Meeting, San Diego, CA, US|USA. (December 2015)

"Characterization of Streptomyces griseoflavus cofilin, a prokaryotic member of the eukaryotic ADF/Cofilin family of proteins." Presented at 2015 ASCB/IFCB Meeting, San Diego, CA, US|USA. (December 2015)

"Anti-chaperon activity of human defense peptides against bacterial and viral effector proteins." Presented at 17th European Workshop on Bacterial Protein Toxins (ETOX17), Braga, PT|PRT. (June 2015)

"ACD-crosslinked actin oligomers are potent toxins inhibiting formin-mediated actin polymerization." Presented at Buckeye Cell Biology meeting sponsored by ASCB, Columbus, OH, US|USA. (April 2015)

"Defensins act as anti-chaperones against thermodynamically unstable bacterial toxins." Presented at Gordon Research Conference, Pisa, IT|ITA. (May 2015)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at University of Maryland, Baltimore, MD, US|USA. (September 2015)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at CMIB program seminar, Columbus, OH, US|USA. (February 2016)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at Department of Chemistry, Riverside, CA, US|USA. (February 2016)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at Science Café program, Columbus, OH, US|USA. (February 2016)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at Department of Chemistry, Cincinnati, OH, US|USA. (March 2016)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at Department of Microbiology, Immunology and Molecular Genetics, Los Angeles, CA, US|USA. (February 2016)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at CMIB symposium, Columbus, OH, US|USA. (April 2016)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at Department of Chemistry and Biochemistry, Eugene, OR, US|USA. (April 2016)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at Dept. of Biochemistry and Molecular Genetics, Charlottesville, VA, US|USA. (October 2016)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at Department of Chemisty and Biochemistry, Oxford, OH, US|USA. (March 2016)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at Dept. of Biochemistry and Biochemistry, Columbus, OH, US|USA. (September 2016)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at Perelman School of Medicine, Philadelphia, PA, US|USA. (June 2016)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at Department of Biochemistry, Biophysics, & Molecular Biology, Ames, IA, US|USA. (February 2016)

"Invited Finalist Talk." Presented at Burroughs Welcome Fund Path finals talk, Research Triangle Park, NC, US|USA. (April 2016)

"Finding "weak spots" for making "strong points" in host-pathogen combats." Presented at School of Medicine, Department of Cell Biology, Syracuse, NY, US|USA. (April 2016)

"Destabilization of pathognic effector molecules by human immune peptides." Presented at Gordon Research Conference, Antimicrobial peptides, Ventura, CA, US|USA. (February 2017)

 

Professional Activities

present Biophysical Society meeting - Motility minisymposium. Biophysical Society. Long Beach, CA.
2011 - present Letters of recommendation.
 

Unpublished Works

Kudryashova E, Koneru PC, Kvaratskhelia M, Strömstedt AA, Lu W, Kudryashov DS. Thermodynamic instability of viral proteins is a pathogen-associated molecular pattern targeted by human defensins. June 2016.

Kudryashova E, Seveau S, Lu W, Kudryashov DS. Invited Review: 'Bacterial toxins: Selective defensin-based inactivation mechanisms'. January 0.

Yehl J, Kudryashova E, Heisler D, Kudryashov DS, Polenova T. Human Cofilin-2 Interface on F-Actin: Atomic-Resolution Insights from Magic Angle Spinning NMR Spectroscopy. January 0.

Schwebach C, Wang C, Golestany B, Kudryashova E, Kloczkowski A, Sirotkin V, Sotomayor M, Kudryashov DS. Bacterial cofilin from Streptomyces griseoflavus shares actin-binding properties with eukaryotic cofilins. January 0.

Harterink M, Esteves da Silva M, Will L, Turan J, Ibrahim A, Lang A, van Battum EY, Aktories K, Pasterkamp RJ, Kapitein LC, Kudryashov DS, Barres BA, Hoogenraad CC, Zuchero JB. DeActs: A genetically encoded toolkit for perturbing the actin cytoskeleton in single cells. June 2016.