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Chad Rappleye, PhD


Staff photo

484 W 12th Ave
540
Columbus, OH 43210

Phone: (614) 247-2718

Email: rappleye.1@osu.edu

 

Current OSU Appointments

Associate Professor, Microbiology Admin

Associate Professor, SBS-Microbial Infectn/Immunity

 

Advising Narrative

Dean's Undergraduate Research Award (2007)Dean's Undergraduate Research Award (2008)Mayers Summer Research Internship (2008)Student Government Academic Enrichment Grant (2008)College of Arts and Sciences Honors Undergraduate Research Scholarship (2008)NIH Intramural Research Training Award (2009) Dean's Undergraduate Research Award (2007) College of Arts and Sciences Honors Undergraduate Research Scholarship (2011)

Academic Advising

2007 - present Eric Holbrook The Ohio State University. Discovery of virulence factors in the extracellular proteome of Histoplasma capsulatum
2008 - present Jessica Edwards The Ohio State University. Graduated 2006.
2009 - present Mohamed Ali The Ohio State University. Comprehensive study of the genetic determinants of virulence and fitness of Salmonella typhimurium in gnotobiotic mice
2010 - present Olga Zemska The Ohio State University. Genes required for intracellular growth of Histoplasma capsulatum
2007 - 2008 Kimberly Roth The Ohio State University. Graduated 2008. Immune response to primary aerosol infection with Francisella novicida
2007 - 2009 Brian Bell The Ohio State University. Graduated 2009. Regulation of virulence gene transcripts by the Francisella orphan response regulator PmrA: role of phosphorylation and evidence of MglA/SspA interaction
2009 Patrick Reville The Ohio State University. Graduated 2009. Targeting phosphoinositol 3 kinase gamma in the treatment of cutaneous leishmaniasis caused by Leishmania mexicana
2009 Jessica Dyszel The Ohio State University. Graduated 2009. What turtles taught us: phenotypes of Salmonella SdiA in animals
2007 - 2011 Jessica Spears The Ohio State University. Graduated 2011. Trypanosoma brucei tRNA editing deaminase: conserved deaminase core, unique deaminase features
2007 - 2011 Michelle Brooks The Ohio State University. Graduated 2011. Nucleotide-binding oligomerization domain containing 2 characterization and function during Mycobacterium tuberculosis infection of human macrophages
2011 Steven Billups The Ohio State University. Graduated 2011. Gene duplication of a Pax-2/5/8 family transcription factor in Caenorhabditis
 

Chapters in Books

Rappleye CA. "Histoplasma capsulatum." In Cellular and Molecular Biology of Filamentous Fungi. Edited by Borkovich KA, Ebbole DJ. 736-750. Washington, DC: ASM Press, January 2010.

Zemska O; Rappleye CA. "Agrobacterium-mediated insertional mutagenesis in Histoplasma capsulatum." In Host-Fungal Interactions: Manipulation of Fungal Gene Expression. Vol. 845. Edited by Brand A; MacCallum D. -. New York, NY: Humana Press, January 2011.

Youseff BH; Rappleye CA. "RNAi-based gene silencing using a GFP sentinel system in Histoplasma capsulatum." In Host-Fungal Interactions: Manipulation of Fungal Gene Expression. Vol. 845. Edited by Brand A; MacCallum D. -. New York, NY: Humana Press, January 2011.

 

Degrees

1995 B.S., University of Utah

2002 Ph.D., University of California San Diego

 

Journal Articles

Rappleye CA; Engle JT; Goldman WE. "RNA interference in Histoplasma capsulatum demonstrates a role for alpha-(1,3)-glucan in virulence.." Molecular Microbiology. Vol. 53, no. 1. (July 2004.): 153-165.

Marion CL; Rappleye CA; Engle JT; Goldman WE. "An alpha-(1,4)-amylase is essential for alpha-(1,3)-glucan production and virulence in Histoplasma capsulatum.." Molecular Microbiology. Vol. 62, no. 4. (November 2006.): 970-983.

Rappleye CA; Goldman WE. "Defining virulence genes in the dimorphic fungi.." Annual Review Of Microbiology. Vol. 60, (January 2006.): 281-303.

Rappleye CA; Eissenberg LG; Goldman WE. "Histoplasma capsulatum alpha-(1,3)-glucan blocks innate immune recognition by the beta-glucan receptor.." Proceedings Of The National Academy Of Sciences Of The United States Of America. Vol. 104, no. 4. (January 2007.): 1366-1370.

Krajaejun T; Gauthier GM; Rappleye CA; Sullivan TD; Klein BS. "Development and application of a green fluorescent protein sentinel system for identification of RNA interference in Blastomyces dermatitidis illuminates the role of septin in morphogenesis and sporulation.." Eukaryotic Cell. Vol. 6, no. 8. (August 2007.): 1299-1309.

Almeida AJ; Carmona JA; Cunha C; Carvalho A; Rappleye CA; Goldman WE; Hooykaas PJ; Leão C; Ludovico P; Rodrigues F. "Towards a molecular genetic system for the pathogenic fungus Paracoccidioides brasiliensis.." Fungal Genetics And Biology: FG & B. Vol. 44, no. 12. (December 2007.): 1387-1398.

Rappleye CA; Goldman WE. "Fungal stealth technology.." Trends In Immunology. Vol. 29, no. 1. (January 2008.): 18-24.

Holbrook ED; Rappleye CA. "Histoplasma capsulatum pathogenesis: making a lifestyle switch.." Current Opinion In Microbiology. Vol. 11, no. 4. (August 2008.): 318-324.

Youseff BH; Dougherty JA; Rappleye CA. "Reverse genetics through random mutagenesis in Histoplasma capsulatum.." BMC Microbiology. Vol. 9, (November 2009.): 236-246.

Rappleye,C. "Functional genomics in Histoplasma." MYCOSES. Vol. 52, (January 2009.): 5-5.

Edwards JA; Alore EA; Rappleye CA. "The yeast-phase virulence requirement for alpha-glucan synthase differs among Histoplasma capsulatum chemotypes.." Eukaryotic Cell. Vol. 10, no. 1. (January 2011.): 87-97.

Holbrook ED; Edwards JA; Youseff BH; Rappleye CA. "Definition of the extracellular proteome of pathogenic-phase Histoplasma capsulatum." Journal Of Proteome Research. Vol. 10, no. 4. (April 2011.): 1929-1943.

Edwards JA; Zemska O; Rappleye CA. "Discovery of a role for Hsp82 in Histoplasma virulence through a quantitative screen for macrophage lethality.." Infection And Immunity. Vol. 79, no. 8. (August 2011.): 3348-3357.

Edwards JA; Rappleye CA. "Histoplasma mechanisms of pathogenesis - one portfolio doesn't fit all.." FEMS Microbiology Letters. Vol. 324, no. 1. (November 2011.): 1-9.

 

Presentations

"Role of Hsp82 in Histoplasma capsulatum pathogenesis." Presented at OSU Department of Microbiology Annual Retreat, Columbus, OH. (January 2010)

"Histoplasma capsulatum virulence gene discovery via a novel high-throughput macrophage assay." Presented at Midwest Microbial Pathogenesis Conference, Ann Arbor, MI. (January 2011)

"Discovery of genes contributing to Histoplasma capsulatum virulence via a novel high-throughput macrophage assay." Presented at Center for Microbial Interface Biology Retreat, Columbus, OH. (January 2011)

"Characterization of an insertional mutagen for Histoplasma capsulatum." Presented at Denman Undergraduate Research Forum, Columbus, OH. (January 2011)

"Histoplasma factors combating host-derived oxidative stress." Presented at Midwest Neglected Infectious Diseases Meeting, Notre Dame, IN. (January 2011)

"Forward and reverse genetic approaches to virulence factor discovery in Histoplasma capsulatum." Presented at XI International Paracoccidiodomycosis Meeting, Sao Paulo, BR|BRA. (January 2011)

"Extracellular virulence factors of Histoplasma capsulatum." Presented at Immunology and Infectious Diseases Departmental Seminar, Cincinnati, OH. (January 2011)

"Virulence factor discovery in the Histoplasma secreted proteome." Presented at American Society for Microbiology 2011 General Meeting, New Orleans, LA. (January 2011)

"Histoplasma extracellular defenses to host-derived oxidative stress." Presented at Midwest Microbial Pathogenesis Conference, Ann Arbor, MI. (January 2011)

"Dectin-1 avoidance by the fungal pathogen Histoplasma capsulatum." Presented at Kowlette Seminar, Columbus, OH. (January 2011)

"Histoplasma capsulatum extracellular defenses to host-derived oxidative stress." Presented at Keystone Symposia - Fungal Pathogens: From Basic Biology to Drug Discovery, Santa Fe, NM. (January 2012)

"Dectin-1 avoidance by chemotype I Histoplasma yeast." Presented at Keystone Symposia - Fungal Pathogens: From Basic Biology to Drug Discovery, Santa Fe, NM. (January 2012)

 

Professional Activities

2010 - present Microbiology Representative. Major Fair Exploration Program for Undergraduate Students. Columbus, OH.
2011 - present Microbiology Representative. Major Fair Exploration Program for Undergraduate Students. Columbus, OH.
2011 - present Presentation Judge. Denman Undergraduate Research Forum. Columbus, OH.
2011 - present Study Section Member (ad hoc). NIH / NIAID.
2011 - present Study Section Member (ad hoc). American Heart Association.
2011 - present College of Arts and Sciences Open House. College of Arts and Sciences. Columbus, OH.
2011 - present Advisory Committee. Midwest Neglected Infectious Disease Meeting.
2012 - present Study Section Member (ad hoc). American Heart Association.
2012 - present Grant Reviewer (ad hoc). North Carolina Biotechnology Center.